I am trying to design primers for a single stranded DNA virus.
But I am having a dilemma as whether we design the primers same way as double stranded DNA?
do we have to design forward and reverse primers both or just a single primer forward or reverse works?
If you've e.g. a parvovirus you design primers as normal, i.e. must be a pair of primers as for double stranded DNA. Albeit there's only one strand present after the first round of PCR there both strands will be present and without the reverse primer the PCR can't work. If you want evidence of this, you've had a SARS-CoV-2 diagnosis? Well thats a forward and reverse primer PCR starting with ssRNA converted to cDNA, i.e. single copy and then a pair of primers PCR the resulting locus.