I would like to know the steps of this question and how to determine the forward and the reverse primers and also the length of the sequence, without using NCBI. If you could explain and give an answer step by step I would appreciate it. Thank you
Without giving too much of the "what they want you to do yourself" bits of this away, here are a few explanations of the image that may help you answer this question:
- As mentioned in the text, the forward and reverse primers are underlined in the image.
+symbols below the forward-direction sequence help determine the positions of the bases above and below. A
+appears every 10 bases.
- The first base in the sequence is at position 1441 (as indicated by the numbers) to the left of the sequence. The first
+to the right of that first base represents position 1450.
- Primers are usually represented as a sequence in a "5-prime to 3-prime direction", which is almost always the left to right direction of the top sequence in an image like this, and the right to left direction of the bottom sequence.
- The length of a PCR product includes the primer sequence.
For help beyond that explanation... this looks like a canned question. Unless you give more context around what bioinformatics project you're working on and how this question relates to your project (i.e. explain why it's a real-world problem rather than a text-book question), I'm going to leave answers to the specific questions asked as an exercise to the reader.