I'm a newcomer to the field of antibodies, and I'm seeking guidance on obtaining 3D structures for my molecular simulations. After browsing the PDB databank, I noticed that there are Fab and Fc structures available. My specific interest lies in investigating the structural dynamics in the entire antibody (AB) and on the hinge region.

I have a few questions:

  1. Is it common practice to attempt building the entire antibody structure and hinge regions from PDB files for fragments, or are there recommended alternatives?

  2. Are there comprehensive PDB structures available for different IgG and therapeutic antibodies that include information on the whole structure and hinge regions? Especially for Bevacizumab and Rituximab?

  3. Does it make sense to select single segments, e.g., CH1, VH, VL, for analysis? Would one expect these structures to be reasonably stable?

Additionally, I'd appreciate any insights into common pitfalls and any recommendations you might have for someone new to this field.

Thank you in advance for your time and assistance.

Best regards, Albert

  • 1
    $\begingroup$ Please edit the question to limit it to a specific problem with enough detail to identify an adequate answer. $\endgroup$
    – Community Bot
    Nov 20 at 21:11
  • 1
    $\begingroup$ Hi, if you've got multiple questions, it's often better to split them up into separate question posts. However, exploratory posts like this are almost always going to produce better outcomes on discussion websites like reddit $\endgroup$
    – gringer
    Nov 21 at 7:21
  • $\begingroup$ wait for cryomicroscopist to deliver nature.com/articles/s41467-022-34090-2 Here, we use cryo-EM to image the structure of the human full-length IgM pentamer, r $\endgroup$
    – pippo1980
    Nov 21 at 19:56


Your Answer

By clicking “Post Your Answer”, you agree to our terms of service and acknowledge that you have read and understand our privacy policy and code of conduct.

Browse other questions tagged or ask your own question.