I want to benchmark my DNA sequencing pipeline. In order to do that I want some gold-standard files in every step(.vcf,.bam,.fastq). I want to generate/simulate a bam file of reads for a given set of vcf. How to do that?
The GIAB project has gold standard vcf, bam, and fastq. But they are not perfect for pipeline testing. Their size is quite big. Also, they are real data. My question was how to generate simulated Bam from vcf.