I'm currently working with data from a Luminex multiplex assay. In this assay, the concentrations of 17 different analyte proteins were identified in 12 groups in triplicate. One of these 17 groups was used as the control, and the log2 fold changes were calculated for the analyte concentration of each sample in each group using the average control concentration for that analyte.
However, now I would like to calculate a p-value for the identified fold changes if possible.
My current preliminary idea is to perform the t test for each group compared with the control group for each analyte, but I don't think that my group sizes are large enough for this. My statistical knowledge is lacking, so if there is a better way of calculating these p-values I don't know about it.
Is there a better way of obtaining these p-values?