I am trying to find out that which DNA sequences(in the below) have a similar domain to a specific toxin which is Clostridium botulinum
You can find the fragment of the DNA sequence that belong to that toxin gene below:
gi|27867582 (fragment of the known Clostridium botuninum toxin gene) GTGAATCAGCACCTGGACTTTCAGATGAAAAATTAAATTTAACTATCCAAAATGATGCTT ATATACCAAAATATGATTCTAATGGAACAAGTGATATAGAACAACATGATGTTAATGAAC TTAATGTATTTTTCTATTTAGATGCACAGAAAGTGCCCGAAGGTGAAAATAATGTCAATC TCACCTCTTCAATTGATACAGCATTATTAGAACAACCTAAAATATATACATTTTTTTCAT CAGAATTTATTAATAATGTCAATAAACCTGTGCAAGCAGC
I ran a blastx job over the above sequence which translates nucleotide into protein. Here's the result.
The result tells me that. The particular DNA sequence listed above, belongs to botulinum neurotoxin type E [Clostridium botulinum]
Besides, I am given two DNA sequences:
>Sequence A TCTATCAAGTAGATTATTAAATACTACTGCACAAAATGATTCTTACGTTCCAAAATATGA TTCTAATGGTACAAGTGAAATAAAGGAATATACTGTTGATAAACTAAATGTATTTTTCTA TTTATATGCACAAAAAGCTCCTGAAGGTGAAAGTGCTATAAGTTTAACTTCTTCAGTTAA TACAGCATTATTAGATGCATCTAAAGTTTATACGTTTTTTTCTTCAGATTTTATTAATAC > Sequence B TCCTGGCTCAGGACGAACGCTGGCGGCGTGTGCTTAACACATGCAAGTCGAGCGATGAAG CTTCCTTCGGGAAGTGGATTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTCA AAGTGGGGGATAGCCTTCCGAAAGGAAGATTAATACCGCATAACATAAGAGAATCGCATG ATTTTCTTATCAAAGATTTATTGCTTTGAGATGGACCCGCGGCGCATTAGCTAGTTGGTA
Again, I translated them into proteins. The result are listed respectively(A and B):
The first job tells that, Sequence A belongs to botulinum neurotoxin type F [Clostridium baratii] which means that similar domain with the above botulinum neurotoxin>
Second job tells me that SequenceB belongs to unknown protein [Clostridium botulinum NCTC 2916]. So that means it is not in the same domain with the above botulinum neurotoxin right?