# Finding out proteins that have a similar domain to a specific toxin

I am trying to find out that which DNA sequences(in the below) have a similar domain to a specific toxin which is Clostridium botulinum

You can find the fragment of the DNA sequence that belong to that toxin gene below:

gi|27867582 (fragment of the known Clostridium botuninum toxin gene) GTGAATCAGCACCTGGACTTTCAGATGAAAAATTAAATTTAACTATCCAAAATGATGCTT ATATACCAAAATATGATTCTAATGGAACAAGTGATATAGAACAACATGATGTTAATGAAC TTAATGTATTTTTCTATTTAGATGCACAGAAAGTGCCCGAAGGTGAAAATAATGTCAATC TCACCTCTTCAATTGATACAGCATTATTAGAACAACCTAAAATATATACATTTTTTTCAT CAGAATTTATTAATAATGTCAATAAACCTGTGCAAGCAGC

I ran a blastx job over the above sequence which translates nucleotide into protein. Here's the result.

The result tells me that. The particular DNA sequence listed above, belongs to botulinum neurotoxin type E [Clostridium botulinum]

Besides, I am given two DNA sequences:

>Sequence A
TCTATCAAGTAGATTATTAAATACTACTGCACAAAATGATTCTTACGTTCCAAAATATGA
TTCTAATGGTACAAGTGAAATAAAGGAATATACTGTTGATAAACTAAATGTATTTTTCTA
TTTATATGCACAAAAAGCTCCTGAAGGTGAAAGTGCTATAAGTTTAACTTCTTCAGTTAA
TACAGCATTATTAGATGCATCTAAAGTTTATACGTTTTTTTCTTCAGATTTTATTAATAC

> Sequence B
TCCTGGCTCAGGACGAACGCTGGCGGCGTGTGCTTAACACATGCAAGTCGAGCGATGAAG
CTTCCTTCGGGAAGTGGATTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTCA
AAGTGGGGGATAGCCTTCCGAAAGGAAGATTAATACCGCATAACATAAGAGAATCGCATG
ATTTTCTTATCAAAGATTTATTGCTTTGAGATGGACCCGCGGCGCATTAGCTAGTTGGTA


Again, I translated them into proteins. The result are listed respectively(A and B):

https://blast.ncbi.nlm.nih.gov/Blast.cgi?CMD=Get&RID=Z423E71C015

https://blast.ncbi.nlm.nih.gov/Blast.cgi?CMD=Get&RID=Z42AKVWC015

The first job tells that, Sequence A belongs to botulinum neurotoxin type F [Clostridium baratii] which means that similar domain with the above botulinum neurotoxin>

Second job tells me that SequenceB belongs to unknown protein [Clostridium botulinum NCTC 2916]. So that means it is not in the same domain with the above botulinum neurotoxin right?

• Looks like you've got two identical links for your blast searches – heathobrien Oct 25 '17 at 6:58
• Ups. I edited the question. – asd sd Oct 25 '17 at 14:27
• What's a 'domain' here? Are you talking about protein domains? Domains of life? As in animals, plants, bacteria etc? What proteins were you given? You show DNA sequences here, not proteins. Please edit your question and clarify. – terdon Oct 26 '17 at 8:29