I know there are existing GO slims predefined. But does anyone know how I can create my own collection of GO terms for enrichment analysis?

  • $\begingroup$ I have never created one, but a GO slims is just a list of GO associated with genes, so you just need to download the list of GO and associate them with your list of genes. What have you tried/searched? What do you need that it is not covered in the FAQ? $\endgroup$ – llrs Nov 29 '17 at 8:04
  • $\begingroup$ GO slims are just a subset of the gene ontology graph. Just a group of terms, no genes. We can't help you create one if you don't explain what terms you want and what sort of analysis you want to do. $\endgroup$ – terdon Nov 29 '17 at 8:33
  • $\begingroup$ I have a list of genes enriched in kidney. So I want to get a list of kidney-related terms and see which of them are significantly enriched. $\endgroup$ – Dr_Hope Dec 3 '17 at 8:34
  • $\begingroup$ @Dr_Hope Then what you really want is to test an enrichment in GO related to kidney not create a GO slim. But if you only use a subset of genes and a subset of GOs the test would be biased (AFAIk) if you don't tell the suitable background population of genes and GO terms. $\endgroup$ – llrs Dec 5 '17 at 9:17
  • $\begingroup$ I've already done a canonical GO to check the background. The enriched terms are mostly kidney-related because our sample is a kidney. That's why I need a GO slim to check kidney-only terms. $\endgroup$ – Dr_Hope Dec 12 '17 at 17:27

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