I run the MinION MinKNOW without the live base-calling option. We know there is Metrichor and Albacore to perform base-calling after this process. However, I have not done any base-calling yet.
My question is: Is it possible to use directly the
fast5 files with
poretools to extract a fastq file without doing any previous base-calling process? I tried it and I get an empty fastq file.
reads directory only contains the
fast5 folder and I run:
poretools fastq fast5/ > output.fastq
Any ideas why do I get an empty file? What is the difference between base-called
fast5 and non-base-called
Now I am trying to do base-calling with Albacore to see if I get a fastq file.