I want to identify the most highly expressed genes across several developmental stages of a given insect, in hopes of identifying a protein that can be used to design an insecticide. I have a few doubts :
Several studies have been performed on individual developmental stages and their transcriptome has been published. Should I compare each set of data against the other manually, to find genes that are expressed universally and then target the most highly expressed ones or is there any software / computational method that can be used instead?
Does this strategy make any sense? Is it scientifically sound? Is it right to compare transcriptomes to find commonly expressed genes?
I did try to look at the materials and methods section of a few papers on comparative transcriptomics, but it only says that data was compared, not really how it was. One paper cited VennBLAST, but I wasn't able to find the tool online.