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By reading Bacher & Kendziorski (2016), I found the following statement:

recent droplet technologies are not yet able to accommodate spike-ins

What's the reason why current droplet technologies cannot use spike-ins? By searching through the available methodologies I found this question on 10X community, but still I cannot understand what is the problem created by using spike-ins with droplet technologies.

  1. Bacher, R. & Kendziorski, C. Design and computational analysis of single-cell RNA-sequencing experiments. Genome Biol. 17, 63 (2016).
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I believe it is mostly a cost issue as spike-ins are "expensive" to use given the large number of cells that are/can be sequenced via droplet based methods (as compared to plate-based ones). The 10x link that you provided actually states that this is possible with 10x and Klein et al. used ERCC spike-ins in 953 droplets (Cell. 2015 May 21;161(5):1187-1201. doi: 10.1016/j.cell.2015.04.044.).

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