By reading Bacher & Kendziorski (2016), I found the following statement:
recent droplet technologies are not yet able to accommodate spike-ins
What's the reason why current droplet technologies cannot use spike-ins? By searching through the available methodologies I found this question on 10X community, but still I cannot understand what is the problem created by using spike-ins with droplet technologies.
- Bacher, R. & Kendziorski, C. Design and computational analysis of single-cell RNA-sequencing experiments. Genome Biol. 17, 63 (2016).