Reading Islam et al. (2011):
From each transcript, a single read is obtained, corresponding to a template-switching site located preferentially at the 59 end of the mRNA.
By reading this page I think that a template switching site is an additional set of nucleotides added at the 3' end of the newly synthesized cDNA, that serve to anchor a oligo-nucleotide used by the reverse transcriptase to synthesize the second cDNA strand. It is called template switching because reverse transcriptase switches strand upon reaching this set of nucleotides.
Is it correct?
- Islam, S. et al. Characterization of the single-cell transcriptional landscape by highly multiplex RNA-seq. genome.cshlp.org 21, 1160–1167 (2011).