I have 16S DNA sequences from diversity sequencing, and want to query these against NCBI's 16S rRNA database. If I ultimately want to get taxids for the species that my sequences have, does it make a difference that my sequences are 16S DNA and not RNA? I am using Blast+.
I imagine this is how analysis of 16S sequences normally goes (get 16S DNA sequences, query them against a 16S DB), but does anyone know if Blast+ or the 16S rRNA DB itself allow for DNA queries?
In other words, if I were to blast a sequence of 16S DNA and its exact transcript RNA, would I get the same results?