# Which are the use cases for the methods for DE in Seurat

In Seurat we can specify multiple methods for finding DE genes.

I am wondering when should we use which one, the use cases. As I understand, whole Seurat package is used for single cell sequencing analysis. I ran different methods for my dataset that was produced from brain cells, around 800 cells in each sample, by single cell sequencing. Now I am confused which methods are the most appropriate to use, which results are the most reliable. For single cell sequencing when would you prefer to use one method over the other?

• what methods did you try (all of them?)? Did you compare between them? Did you review the mathematical background and assumptions they make to see if they fit your data?
– llrs
May 11 '18 at 7:01