Hi am trying to align RNA-seq reads using STAR through following commands,
STAR --runMode alignReads --runThreadN 26 --outSAMtype BAM SortedByCoordinate Unsorted --sjdbOverhang 100 --genomeDir wheat --readFilesIn G3_cleaned_R1.fastq G3_cleaned_R2.fastq --sjdbGTFtagExonParentTranscript wheat.gff3
Jun 05 13:53:59 ..... started STAR run
Jun 05 13:53:59 ..... loading genome
Jun 05 14:07:12 ..... started mapping
Jun 05 18:42:47 ..... started sorting BAM
EXITING because of FATAL ERROR: number of bytes expected from the BAM bin does not agree with the actual size on disk: 509186583 0 45
EXITING because of FATAL ERROR: number of bytes expected from the BAM bin does not agree with the actual size on disk: 648593301 0 47
Jun 05 18:42:47 ...... FATAL ERROR, exiting
Jun 05 18:42:47 ...... FATAL ERROR, exiting
EXITING because of FATAL ERROR: number of bytes expected from the BAM bin does not agree with the actual size on disk: 660412146 0 46
Jun 05 18:42:47 ...... FATAL ERROR, exiting
EXITING because of FATAL ERROR: number of bytes expected from the BAM bin does not agree with the actual size on disk: 470931698 0 44
Jun 05 18:42:47 ...... FATAL ERROR, exiting
EXITING because of FATAL ERROR: number of bytes expected from the BAM bin does not agree with the actual size on disk: 653155717 0 43
Jun 05 18:42:47 ...... FATAL ERROR, exiting
Segmentation fault (core dumped)
Although there is much free space on my system as shown below:
> df -Th
Filesystem Type Size Used Avail Use% Mounted on
devtmpfs devtmpfs 63G 0 63G 0% /dev
tmpfs tmpfs 63G 136K 63G 1% /dev/shm
tmpfs tmpfs 63G 1.9M 63G 1% /run
tmpfs tmpfs 63G 0 63G 0% /sys/fs/cgroup
/dev/sda3 ext4 3.6T 1.4T 2.2T 39% /
/dev/sda2 ext4 9.8G 57M 9.2G 1% /boot
tmpfs tmpfs 13G 0 13G 0% /run/user/480
tmpfs tmpfs 13G 16K 13G 1% /run/user/1000
How this problem can be solved?