# How is prior.count used by edgeR's cpm

edgeR's cpm function has an argument called prior.count. Based on my understanding of the documentation, it is supposed to be adding a fixed number per sample which is proportional to the library size of said sample. Average of all numbers added to all samples would be equal to prior.count.

However, looking at actual data this does not seem to be the case. Given an imaginary data frame of

df = data.frame(a = c(1,2,3,0),c = c(1,2,3,0)*3, b = c(1,2,3,0)*2)


We can try to calculate log cpms by doing

logCPM = cpm(df,log = TRUE,prior.count = .5)


We can also calculate regular cpms by doing

CPM = cpm(df)


If we wanted to see, what is the number that is added to the CPMs before getting logged, we could do

difference = 2^logCPM - CPM


To make it look pretty lets use tibble

tibble::as.tibble(difference)

# A tibble: 4 x 3
a                    c                    b
<dbl>                <dbl>                <dbl>
1  25641                25641                25641
2  12821                12821                12821
3 -    0.0000000000582 -    0.0000000000582 -    0.0000000000582
4  38462                38462                38462


Here we see that the number that is added has no relation to the library size of the sample. What I want to learn is, how is this number that is added to each cpm is calculated based on prior.count.

I have been digging through the code but that part goes into C++ territory which hinders my understanding of what is going on

• I get different results in the difference table. Which version are you using ? (I am in R 3.5.1 and edgeR 3.22.2)
– llrs
Sep 6 '18 at 8:30

The prior count ends up getting scaled by the ratio of a library size to the average library size and then multiplied by 2 before getting added to each library size (I'm sure there's a good reason for that, but I don't know what it is). Using the example df data frame in your post, let's walk step-by-step through what cpm() is doing:

df = data.frame(a = c(1,2,3,0),c = c(1,2,3,0)*3, b = c(1,2,3,0)*2)
prior.count = 0.5
# First, we need to calculate a library size
lib.size = colSums(df)
# Calculate the average library size and the adjusted priors
ave.lib = mean(lib.size)
adjusted.prior = prior.count * lib.size / ave.lib
# Update the library sizes
# Now we can compute the CPM

The matrix transposition looks really ugly in the last step, but it's needed so R adds things like adjusted.prior to each row of df.
• How do you know that edgeR is scaling that way to obtain the adjusted.prior? By the way sweep could be used to avoid having to transpose the matrix
• It's stated in the source code. I know I can use sweep(), but t() takes fewer characters. Sep 6 '18 at 9:46