When I try to import the data using
dds <- DESeqDataSetFromTximport(txi, sampleTable, ~Group + Cre)
I am getting the error:
Error in validObject(.Object) : invalid class “DESeqDataSet” object: levels of factors in the design have non-unique level names after make.names() is applied. best to only uobject letters and numbers for levels of factors in the design
Here is how my
sampleTable looks like:
ID Group Cre 1 Bla_36 IL4 - 2 Bla_38 IL4 - 3 Bla_11 IL4 - 4 Bla_17 IL4 - 5 Bla_20 IL4 + 6 Bla_21 IL4 + 7 Bla_8 IL4 + 8 Bla_22 IL4 + 9 Bla_7 IL4 + 10 Bla_35 PBS - 11 Bla_37 PBS - 12 Bla_13 PBS - 13 Bla_1 PBS - 14 Bla_9 PBS + 15 Bla_23 PBS + 16 Bla_19 PBS + 17 Bla_24 PBS +
What am I doing wrong here? And how to make it working?