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Sorry,

I have analysed my time series single cell data with Scuba algorithm but that says that there is no bifurcations in my data and outputted a single chain of 4 states with no branching like below figure

enter image description here

Sorry, I googled but I am not quite sure what this means

Any suggestion please?

Thanks a lot

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The most obvious interpretation is that the four cell lines are related in a linear pathway rather than a branching one.

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