I'm thinking that this isn't actually possible, but I'd like to check before I write it off. I have RNA-seq data from cells under three different conditions, there are no replicates for any of the conditions. The data has been processed with RSEM, and log2 fold changes have been calculated for each control-test pairing using the normalized expected read counts using EBseq.
If possible, I'd like to also calculate the p-value for each of these fold-changes, however, because there are no replicates I don't think that this is possible. Is there any way to do this?