Supposing upcoming RNA-seq for 3 conditions and 3 replications for each, how much is data storage requirements?
The breakdown would be:
- 1.5-2 GB per sample for fastq files.
- ~3GB per BAM file, depending on the aligner
So ~5GB per BAM file, which is about 50GB total. That can vary by maybe 20%, depending on how compressible the results are (I rearrange our fastq files to make them more compressible, so I'm probably underestimating what others would see).
Obviously using Salmon/Kallisto would halve the storage requirements. Using CRAM would also decrease things, though obviously fewer tools support it.