0
$\begingroup$

I have followed the Seurat Tutorial:Integrating stimulated vs. control PBMC datasets to learn cell-type specific responses by using my data. And I am confused about how to write the script to calculate the cell number of each sample on each cluster.

I have run TSENPlot to know the samples and I'm using another TSENPlot to get the clusters. If I want to know find the cluster1 of each sample's number how can I write the script?

enter image description here

$\endgroup$
1
  • 1
    $\begingroup$ Please edit your question and give us some more details. What exactly do you need? Can you show us the code you have run? $\endgroup$
    – terdon
    Mar 5, 2019 at 10:22

1 Answer 1

2
$\begingroup$

Assuming you are following the basic seurat guide:

pbmc@ident

You can access the 'ident' slot of your Seurat object and you will get a vector of the cluster IDs and the names will be the respective cells.

head(seuratobj@ident)
Cell1A7 Cell1C4 Cell1D1 Cell1D2 Cell1D3 Cell1D5
0 3 3 1 3 1 
Levels: 0 1 2 3 4
$\endgroup$
1
  • $\begingroup$ In my example, the printing wasn't great but essentially Cell1A7 is in cluster 0, Cell 1C4 is in cluster 3, etc... $\endgroup$
    – story
    Mar 5, 2019 at 10:09

Your Answer

By clicking “Post Your Answer”, you agree to our terms of service and acknowledge that you have read and understand our privacy policy and code of conduct.

Not the answer you're looking for? Browse other questions tagged or ask your own question.