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I've done my 1st trial of 1D^2 whole genome sequencing using LSK-SQK308 kit with R9.5 flowcell. Without doing library fragmentation, I encountered the unexpected library shearing during sequencing.

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But I didn't see significant portion of low-molecular-weight fragments in the QC gel photo before and after library preparation.

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Anyone using nanopore has encountered this before? Any explanation? And how to avoid it? I could not find evidence of the problem in my QC steps...

Thanks in advance!

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