I've done my 1st trial of 1D^2 whole genome sequencing using LSK-SQK308 kit with R9.5 flowcell. Without doing library fragmentation, I encountered the unexpected library shearing during sequencing.
But I didn't see significant portion of low-molecular-weight fragments in the QC gel photo before and after library preparation.
Anyone using nanopore has encountered this before? Any explanation? And how to avoid it? I could not find evidence of the problem in my QC steps...
Thanks in advance!