# Why do I have >10,000 cells in the 10X matrix produced by cellranger?

In my scRNA seq experiment, single-cell libraries were generated using the GemCode Single-Cell Instrument and Single Cell 3′ Library & Gel Bead Kit v2 and Chip Kit (10x Genomics) according to the manufacturer’s protocol. About 8700 cells were added to each channel with a targeted cell recovery estimate of 5000 cells.

However, after processing my raw data with CellRanger software, I get 737,280 cells (barcodes) and about 31,000 genes. I cannot understand why I get so many cells and why this barcode whitelist is not up to 10,000 cells. After filtering this data retaining genes expressed in >= 5 cells as well as retaining cells with at least 200 detected genes, I get around 2200 cells.

Thanks

The raw data from cell ranger contains all of the barcodes detected in the experiment. These raw barcodes are filtered by cell ranger to identify the barcodes that likely represent cells rather than empty droplets / dead cells. You can find the filtered data in the filtered feature-barcode matrices. You can also examine the Run summary HTML file to see a summary of the cell ranger barcode filtering.