I have single cell data which I have analysed for differential expression. In my experiment, I subjected two groups of mice (a control and a treatment group) to treatment that will lead to the proliferation of my cells of interest in the treated mice. Other scientists have said that the increase of cell numbers caused by this treatment results from increased recruitment from precursor cells from the bone marrow while other scientists argue that the increase in cell number is due to local proliferation.
I wish to address this question computationally, based on my single cell rna seq data using R or any other method. I have googled but I can find no suitable tool. Does it suffice to test the expression of known cell migration markers, produce a migration score and judge based on the migration scores of my proliferating cells?
If not, I will be very grateful for suggestion of a computational tool, method or package that can help me address this question even indirectly.
Thank you in advance.