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If I have 2 samples hashed, ran together and generated one ADT file for them, then how do I plot 2 different ADT clusters separated based on the hashtags. I did use HTOdemux, but it shows me population of the hashtags only. I want to get ADT count files separately for those 2 hashed samples.

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  • $\begingroup$ It seems like you got some new data in and we are very happy to help you get started with your analysis. However, without some details on what you have tried and what went wrong it is very hard for us to tell you what to do. When you are using HTO demux I assume you are working with citeseq count and this vignette: satijalab.org/seurat/v3.0/hashing_vignette.html. Please update your question and add the codes for the steps that give u unexpected results $\endgroup$ – Mack123456 Aug 24 at 1:47

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