Questions tagged [1d2-reads]
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3
questions
6
votes
1answer
35 views
Help with 1D^2 library shearing
I've done my 1st trial of 1D^2 whole genome sequencing using LSK-SQK308 kit with R9.5 flowcell. Without doing library fragmentation, I encountered the unexpected library shearing during sequencing.
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1
vote
1answer
168 views
Basecalling process carried out by full_1dsq_basecaller.py/.exe
I am unable to understand what exactly the full_1dsq_basecaller.py/.exe script does. Is it giving the consensus sequence for linear and complementary strand, or it is just giving the reads that came ...
3
votes
1answer
350 views
Removing repeated reads from nanopore 1D² reads
This is summary of discussions and a question that was posted by Devon O'Rourke on Twitter
Following Albacore basecalls on a 1D² library I get two sets of .fq files, summary stats, etc.: one for the ...
