Questions tagged [base-calling]
The process of transforming the raw signal of a sequencer (light, images, electrical signal) into DNA or RNA bases. For Illumina, the basecalling consists of transforming the pictures with lights of different colours into bases. For Nanopore, it consists in transforming the electrical signal into bases. Basecalling can also include the calculation of the probability of the called base being correct (PHRED score). Albacore is a basecaller for Nanopore data.