Questions tagged [bed]
Browser Extensible Data, a collection of related plain text formats for describing genome features for visualization with genome browsers.
35
questions
2
votes
2answers
79 views
Efficiently iterate over bed and subset pyranges objects
I am using pyranges and I have two datasets that I want to compare in several genome-wide intervals based on a bed file.
For every interval in the bed file, I want to get all overlapping positions ...
1
vote
1answer
46 views
BED file from .bam alignment structure
I am using a pipeline from nanopore to detect structural variants (SVs) in a human sample with long-reads sequencing. The first steps of the pipeline are:
index the reference genome with ...
2
votes
1answer
23 views
UCSC liftover cannot produce bed format output
I was trying to use UCSC liftover tool to convert human coordinates to mouse. While most of the files worked, one file didn't:
...
1
vote
0answers
201 views
Making a bed file for RSeQC
I making a bed file for RSeQC, so it can do things like compute the number of reads from exons, introns, 5"UTRs, etc.
I want to use a bed file that corresponds to my GTF file, so I use gtf2bed to ...
1
vote
0answers
140 views
bedtools single-nucleotide coverage in BED-specified regions for multiple BAMs
I have a BED6 (BED + name, score, strand info) file that defines some regions of interest. I also have a set of BAMs corresponding to different samples. I would like to obtain output similar to <...
2
votes
5answers
1k views
How to create a .bed file from .fasta? [duplicate]
I have some problems with creating a .bed file for hg19 which I will be able to load on IGV.
The fasta file contains rows of this form:
...
0
votes
0answers
38 views
From bedtools “genomecov” back to .bed
I have the results of:
bedtools genomecov -d...
Output:
0
0
10
10
10
20
20
And i want to convert it back to bed file.
e....
6
votes
3answers
187 views
Fast filtering of intervals not falling within a certain distance from known genes
I would like to filter a bed file with intervals, ie. in the format of:
chr1 13800 14301
chr1 15500 16001
chr1 19400 19901
chr1 22800 23301
...
6
votes
1answer
101 views
Generating random BED intervals given constraints
Problem is to generate a random BED interval given the following constraints:
minimum start
maximum end
fixed length
maximum ...
3
votes
1answer
43 views
2
votes
1answer
454 views
How to split bedfile into non-overlapping regions and compute aggregation function on duplicate segments
I have a bed-like file in which I have several overlapping regions with associated features. I want to split the overlapping regions into disjoint regions in order to process the file (e.g. with ...
4
votes
2answers
235 views
How can I easily get the read size distribution of reads mapping on a certain set of regions?
Suppose I have a BAM file indicating where reads in a library have mapped, and a bed file describing a set of genomic regions.
Is there a way to easily get the size distribution of the reads mapping ...
3
votes
2answers
138 views
Filter bam using SNP list in bed format with minimum mapping quality and base quality
I have a bam file and a bed file that defines a list of SNPs. I would like to filter the bam file to contain only those reads with a minimum mapping quality that overlap at least one SNP with a ...
7
votes
6answers
870 views
bed file with N regions of GRCh38 reference?
How do I obtain a bed file with the list of N-regions of GRCh38 reference? This is, the regions where the sequence is a stretch of Ns.
2
votes
1answer
315 views
bed file merge book-end features only if same name in column 4
I have a bed file where some entries have the same name in column 4 but are book-ended in coordinates, e.g. see below:
...
1
vote
3answers
1k views
Finding the nearest gene to a specified gene region
I've got some data on genomic regions of interest in a bedgraph-like format from the Mus musculus [mm10] genome, and would like to find out the nearest gene to these regions.
Some of the regions will ...
0
votes
2answers
235 views
bed entries collapse based on score column
I have a bed file where some entries have the exact same chr:start-end but change in the name and score column. I would like to apply bedtools or a similar tool to obtain a filtered file where for ...
2
votes
2answers
249 views
Parse out exon coordinates from bed file for each gene
This is my bed file for all the exon coordinates I want to take out all the exons of a given gene. Let say I have gene in chr 1 which starts from chr1 11868 12227 ...
2
votes
2answers
824 views
bigWig to bed for regions above/below threshold
I'd like to threshold a bigWig file on a value and get all regions above or below that value in bed format. Ideally without too many file conversions. Are there any tools to do that?
8
votes
2answers
934 views
5'UTR and 3'UTR annotation in yeast
I am working on a project in which I need to compute several parameters (such GC content and length) of 5'UTR and 3'UTR sequences of Saccharomyces cerevisiae yeast genes.
The problem is finding a ...
7
votes
2answers
101 views
How to release an R package with several bed files?
I'm currently creating an R package, and my scripts require before any analysis is done that 1-2 bed files be loaded. Normally, I would run the scripts with the following:
...
1
vote
1answer
79 views
TruSeq Exome Kit bed file old version
I have to run an analysis using Whole Exome Sequencing (WXS) data prepared with the TruSeq Exome Kit (2012).
I need the .bed file for the kit and I found it here.
...
2
votes
1answer
238 views
Dealing with indels when converting a position to chromStart and chromEnd for a bed file
Starting with a chromosome # and position, I am trying to get chromStart and chromEnd values for a .bed file, but I am not sure how to calculate chromEnd when I have a variant that is an insertion or ...
6
votes
4answers
329 views
Any fast options to query large VCF bed intervals?
I'm doing some analysis and I need to subset a large VCF file (~8GB gziped) given a bed interval and identify within a subset of rsid.
Unfortunately, both my normal choices to do this analysis (<...
6
votes
5answers
1k views
11
votes
4answers
2k views
How to convert BED to GFF3
I would like to convert a BED format to GFF3.
The only useful tool that I could find via a google search seems to be Galaxy, ...
6
votes
2answers
534 views
How can I compare two bed files?
I am trying to translate (lift over) bed files describing genomic regions from hg37 to hg38. I have tried both UCSC's LiftOver tool and CrossMap but saw that they give me different results. I ...
11
votes
3answers
4k views
How to obtain .bed file with coordinates of all genes
I want to get a .bed file with the genes' names and canonical coordinates, also I would like to have coordinates of exons, too. I can get the list from UCSC, however, if I choose UCSC Genes - ...
5
votes
4answers
633 views
Range overlap python error with genomic regions
I have two files
s3.txt :
1 10 20
1 5 20
2 20 30
2 25 30
1 10 50
2 20 60
1 14 17
s4.txt:
1 10 20
2 20 30
I am trying to match col0 ...
3
votes
2answers
166 views
After artificially creating events in a FASTA file, how do I keep track of the old coordinates?
I'm beginning with the reference genome in FASTA format, hg19. I am reading the sequence into a Python dictionary with BioPython:
...
9
votes
4answers
194 views
Annotation format design
Bashing file formats is a favorite pastime in bioinformatics, and annotation file formats such as GFF and BED seem to get special attention. A lot of this frustration stems from community's shockingly ...
6
votes
1answer
50 views
What's the scaling for HOMER metagenes?
I'm trying to use HOMER to make a metagene profile over gene bodies using a bedgraph file I've generated. The problem is that every time I do, I get really weird scaling on the y-axis. I should be ...
6
votes
1answer
363 views
17
votes
7answers
5k views
16
votes
3answers
2k views
Convert a BAM file from one reference to another?
I have a set of BAM files that are aligned using the NCBI GRCh37 human genome reference (with the chromosome names as NC_000001.10) but I want to analyze it using a BED file that has the UCSC hg19 ...
