Skip to main content

Questions tagged [crispr]

For questions about the CRISPER-Cas9 genome editing technology

4 questions with no upvoted or accepted answers
Filter by
Sorted by
Tagged with
2 votes
1 answer

Read alignment using Bowtie2

So this is related to CRISPR-CAS9. I am working with off-target predictions for my thesis and was looking at all scientific papers related to CRISPR. I found one and decided to use their datasets. The ...
Ina's user avatar
  • 21
2 votes
0 answers

How to analyze and visualize CRISPR-CAS9 screening results?

I have a whole-gene CRISPR screening of an assay at different time points. I created a table of the gene number and the count of its reads: ...
0x90's user avatar
  • 1,437
1 vote
0 answers

Which components must be included in the HDR plasmid?

I am trying to design a plasmid for our knock in experiment and wanted to clarify some technical issues. I wanted to know exactly which components must be included in the plasmid. I know that I have ...
Research HMBR's user avatar
1 vote
0 answers

CRISPR Screen Control - What genes do not affect phenotype when knocked out?

I have some raw read count data from a crispr screen. The library used was addgene's Mouse Improved Genome-wide Knockout CRISPR Library v2 I'm following an analysis pipeline that requires counts ...
geom_na's user avatar
  • 237