Questions tagged [crispr]
For questions about the CRISPER-Cas9 genome editing technology
4
questions with no upvoted or accepted answers
2
votes
1
answer
253
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Read alignment using Bowtie2
So this is related to CRISPR-CAS9. I am working with off-target predictions for my thesis and was looking at all scientific papers related to CRISPR. I found one and decided to use their datasets. The ...
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2
votes
0
answers
94
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How to analyze and visualize CRISPR-CAS9 screening results?
I have a whole-gene CRISPR screening of an assay at different time points. I created a table of the gene number and the count of its reads:
...
- 1,417
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0
answers
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Which components must be included in the HDR plasmid?
I am trying to design a plasmid for our knock in experiment and wanted to clarify some technical issues.
I wanted to know exactly which components must be included in the plasmid.
I know that I have ...
1
vote
0
answers
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CRISPR Screen Control - What genes do not affect phenotype when knocked out?
I have some raw read count data from a crispr screen.
The library used was addgene's
Mouse Improved Genome-wide Knockout CRISPR Library v2
I'm following an analysis pipeline that requires counts ...
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