Questions tagged [cutadapt]
A command-line tool used to remove adapter sequences from raw sequencing reads. Can also be used to trim low-quality bases from the ends of reads.
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Bowtie2 gets stuck on alignment
I am aligning a fastq file as follows:
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working with cutadapt
I'm working with ion torrent data where I apply the program cutadapt.
I'm analyzing ITS seq data, as well as Matk.
When I'm using cutadapt, I search the information for this genes, and built new ...
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Removing adapters + primers from fastq files
I am currently in the process of removing adapters and primers from some 16S data acquired, and have a conceptual question regarding this pre-processing step:
So there are a series of different primer ...
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Why is cutadapt discarding some paired end reads?
So, I am using cutadapt to remove two primers from some paired end reads.
I have 2 files for each sample, i.e. sample_R1.fastq and sample_R2.fastq.
I have primer_1 and primer_2 and both of them can be ...