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Questions tagged [data-download]

The process of downloading data from the internet.

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2 votes
2 answers
334 views

How can I download multiple SRR* files at a time when using sratoolkit's fastq-dump?

I have multiple SRR files to download, and their accession is in a list: SRR9004325 SRR9004326 SRR9004327 I read the NCBI document but don't find how to achieve ...
0 votes
1 answer
42 views

How to download multiple UniRef clusters with all their members?

We are trying to download multiple UniRef clusters in one batch on the website. However, it seems the members are not downloaded... Could you guide me a way to the solution? Many thanks! For example, ...
1 vote
1 answer
145 views

How can extract the list of genes name from the raw data in GEO?

The format of the raw data that upload in GEO is different, like TXT, CEL or other form. How can I extract the list of genes name from these formats? And then how can I normalize them with R? I would ...
0 votes
0 answers
44 views

ZINC15 database: proxy server error when trying to download catalog in any format

I am a complete beginner in the use of such databases. When navigating to the ZINC15 database website and trying to download an entire catalog with the "Download all as" option the request ...
0 votes
0 answers
9 views

Where would I get the activity profile dataset for Lantus Solostar insulin, and for other diabetes treatments such as Metformin?

I am working on a personal project for a better, more informed management of my own Diabetes Mellitus, Type 2 (aka "T2DM", or "T2D"). With some difficulty I am able to access and ...
-1 votes
1 answer
39 views

Where can you download functional domain labels of the human genome?

I'm visualizing where mutations occur in a gene, and would like to add functional domains to the plots. I have some trouble locating sources to download data on functional domains. The dataset should ...
0 votes
0 answers
28 views

Exporting Panther functional classification of gene list

After inputting a list of gene and running an analysis in https://www.pantherdb.org/ (such as "functional classification"), I get a result table that is limited by an X number of entries ...
4 votes
2 answers
130 views

Where to download a file with major and minor alleles at every position?

I want a list of all variants, i.e. sites which are known to vary between human to human. For example, it should ideally cover all sites in here, but without samples. I don't want a giant reference ...
17 votes
6 answers
5k views

What's the best way to download data from the SRA? Is it really this slow?

I'm trying to download three WGS datasets from the SRA that are each between 60 and 100GB in size. So far I've tried: Fetching the .sra files directly from NCBI's ftp site Fetching the .sra files ...
3 votes
2 answers
5k views

How to download the whole BLAST nt database into a specific folder?

I have successfully downloaded the whole nt BLAST database into the current folder using: wget -b "ftp://ftp.ncbi.nlm.nih.gov/blast/db/nt.??.tar.gz" However, I ...
0 votes
0 answers
41 views

Get BioProject code of a GEO dataset

In publications the GEO code is usually shared and not the BioProject/study code. I want to get the BioProject/Study Accession code of a GEO dataset (to later download the data from ENA). I know that ...
4 votes
1 answer
116 views

What is the NCBI's definition of an "atypical genome"?

Using the new NCBI Datasets platform, you can browse the collection of genomes associated with one or more taxa. For example, searching Pseudomonas aeruginosa returns 19,878 genomes as of 29 March ...
3 votes
4 answers
1k views

How to download ligands for PDB structure

I have some PDB IDs and for every structure, I need all its ligands, so I want to automate the process. The ligands are different for every chain: ...
2 votes
0 answers
109 views

Problem with nextflow -resume and path.fromSRA

I have a problem with nextflow -resume Here is my script : ...
1 vote
0 answers
50 views

Download Addgene database

Is anyone aware of a way to download all vector sequences from addgene at once? As a non-profit, I'd assume they shouldn't be opposed to making this data available, but it doesn't seem to be possible. ...
2 votes
2 answers
2k views

Downloading URL using AWK for fixed fields

I have a text file as follows ...
3 votes
1 answer
89 views

Downloading genes from NCBI in fasta format

I'm pretty new in bioinformatics. I need to download FASTA sequences of several genes. The list of genes I have assembled consists of 140 genes, so I'd rather do this through via code than download ...
2 votes
2 answers
1k views

How to wget a gff from NCBI database?

Say I want to use wget to download a gff file for the X chromosome of release 5.57 of the Drosophila melanogaster genome. The NCBI page with the gff file for just this is here. However, I do not see ...
4 votes
0 answers
44 views

Retrieve protein sequence from Mgnify given only accession code

I only have the accession codes of several proteins from the MGnify database (https://www.ebi.ac.uk/metagenomics/). I would like to retrieve the full amino acid sequence data from the database, but I ...
2 votes
1 answer
103 views

How to get coding sequences (CDS) of UniProt reference proteomes?

I downloaded the following reference proteomes: https://ftp.ebi.ac.uk/pub/databases/reference_proteomes/QfO/ ...
2 votes
1 answer
85 views

How do I download the mitochondrial haplogroup datasets for human genetics online?

I seem to have landed on the mitomap.org site, but I don't know what to make of it or what do with it / how to get the genomes onto my computer. It sounds like the genomes are stored in GenBank, but ...
0 votes
1 answer
2k views

Download or create VCF from Human Genome Diversity Project (HGDP)

I can't seem to find HGDP data in VCF format. Does anyone know where I can download it from? Perhaps there a Snakemake pipeline for calling VCFs from this data?
2 votes
1 answer
108 views

Taking too long to download metadata file (estimated size 30MB) from Covid-19 Data Portal

I am working on developing a tool to classify covid variants. So I am trying to download SARS-CoV-2 sequence data along with lineage annotation from Covid-19 Data Portal (I could not find a better ...
2 votes
2 answers
341 views

Replacing column 1 in file1 with matching ID's in file 2

I have downloaded two files: File1.csv from: https://depmap.org/portal/download/data_slicer/download?file_path=20220227%2F3c335b7d-c18e-4788-b2e6-512d71f0a339%2Fexport.csv&name=Metabolomics.csv ...
3 votes
2 answers
683 views

What happened to sra ftp server?

My whole (professional) life I am downloading sequences from SRA and ENA databases. Long time ago, I used prefetch from sra-tools, but later I switched to simply using wget from ftp servers of the two ...
1 vote
2 answers
47 views

Understanding exercise on file coverage with question on summary statistics

I'm doing an exercise that asks for two files: Input 1: A target file (.bed format) contains multiple regions from ...
1 vote
1 answer
380 views

Where to find publicly available Sanger chromatography data?

I am looking for any public available databases where I can download Sanger chromatography data ideally in Ab1 or SCF file format. I need extremely large amounts of this, since I want to use it for ...
2 votes
1 answer
3k views

How to download data from SRA in Linux systems via the command line?

My workflow for downloading data from SRA has been the following: Access SRA Run Selector. Enter the accession number for the project of interest. Download "Accession List" for the "...
6 votes
7 answers
16k views

Download multiple SRA files

I want to download all SRA file from the following project. Is there a method to download all the SRA files at the same time?
5 votes
4 answers
280 views

How do you query and explore ENCODE data?

I am looking for a modular way to query data from ENCODE. For example, I would like to get CHiP-seq or similar tracks for a specific cell line. What's the proper way to do it? Finally, is there an ...
1 vote
3 answers
5k views

Download multiple fastq files using fastq-dump

I want to download the following fastq files at the same time in Salmon: - SRR10611214 - SRR10611215 - SRR10611215 - SRR10611216 - SRR10611217 Is there a way ...
1 vote
0 answers
207 views

Trying to download a large number of files from ENA programmatically

I am downloading a large number of files from the ENA in Python using Multithreading. As an example, to be more concrete, I tried downloading 12 files simultaneously using 12 threads. I noticed that ...
4 votes
2 answers
859 views

Downloading all COI sequences from BOLD fails

I have metabarcoding sequence data (COI) from bulk animal samples (including arthropoda, nematoda, annelida, mollusca) and I want to BLAST all of these sequences. I used following command to do this: <...
0 votes
0 answers
39 views

Blood analysis devices: how and where is the data saved

I've read that there are blood analysis devices that can automatically determine the data for each specified analyse. You can expect to get the results in less than a day. Q: How and where do they ...
2 votes
2 answers
356 views

How to download multiple proteomes at once?

I'm looking for a way to download multiple proteomes at once from one clade, as fasta. Probably from the NCBI, because it looks more user-friendly than Ensembl, but Ensembl is okay too. In the best ...
0 votes
2 answers
246 views

GDC data portal cancer subtype

I need to use Gene Expression quantification data (using HTSeq-FPKM-UQ) for breast cancer data on the gdc portal (https://portal.gdc.cancer.gov/repository) to classify breast cancer subtypes (luminal ...
3 votes
2 answers
270 views

How to link GDC ids to CCLE cell line names?

Hello bioinformaticians, I've recently downloaded few RNAseq data files from Genomic Data Commons Data (GDC) portal. These files belong to Broad institutes CCLE project. Now the problem is that GDC ...
3 votes
1 answer
74 views

How to query the Human Microbiome Project (HMP) to find all subjects with both 16s and WGS workups?

I am looking for a query to run on the HMP database that will return all subjects who have had BOTH 16s and whole genomes sequence (WGS) workups. I am currently using this query... ...
6 votes
4 answers
2k views

Convert SRA to FastA

I'm trying to get the FastA files for some accessions (like NC_001416.1). I did not managed to find an FTP server or direct link to these files (I want to get it from command line with ...
6 votes
1 answer
647 views

Download data from the Human Microbiome Project via ascp

I have asked this question in biostars, but I am trying here as well as people working with "omics" data might be able to help. I think my issue relates understanding how large data storage on online ...
1 vote
1 answer
46 views

Where can I find the DNA sequence of the C. elegans rRNA cluster?

Where can I find the DNA sequence of the C. elegans rRNA cluster? Ideally, I would be able to do it using the command-line. I've tried using NCBI but the results are unsatisfactory. I am interested ...
1 vote
1 answer
477 views

How to download SNP data from specific regions, population and positions?

I am totally new in Bioinformatics and I would like to apply my knowledge in feature selection on the tag SNP problems. To do that, I've read a lot of papers and books in order to understand the main ...
5 votes
2 answers
736 views

A reliable fetcher of short read using SRA/ENA accession

I am trying to build a workflow that gets data automatically from databases of sequencing reads (SRA - sequence read archive, ENA - european nucleotide archive). Till now I was pulling everything from ...
6 votes
1 answer
499 views

How to validate that BAMs have been downloaded correctly?

I currently have several hundred BAM files which were downloaded by someone else. These have remained untouched---before working with them, I would like to double-check that these BAMs have been fully ...
2 votes
1 answer
154 views

SNP data Download Help

I am new to the field of Bioinformatics. Single Nucleotide Polymorphism or SNP data are available on the the Internet to download.SNP related data are valuable for variety of reasons ranging from ...
3 votes
1 answer
288 views

How to download gene expression data from NCBI gene database

In the NCBI gene database, I can add the expression tracks (circled in picture blow) through 'Tracks' button, but How I can download the expression data directly, not just look the picture?
2 votes
1 answer
998 views

How to download the whole directory of an ensembl FTP page?

I am interested in downloading all the files at: ftp://ftp.ensembl.org/pub/release-91/embl/bos_taurus/ I know how to download a single file from that page using wget (Unix) or download.file() (R) ...
4 votes
2 answers
639 views

How to dump genes from GenBank in GFF3 format?

This question has also been asked on BioStars If I look at this record in GenBank I see about 6k genes: https://www.ncbi.nlm.nih.gov/nuccore/CM000760?report=gbwithparts I'd really like to be able to ...
6 votes
1 answer
124 views

Stable download URLs

One big problem that I'm regularly facing is that URLs for downloading Bioinformatics data (e.g., RefSeq releases or NCBI genome releases) disappear. Does anyone have any good solution for this?