Questions tagged [ebseq]

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Questions about EBSeq (in R)

I have made a UMAP of malignant cells and the result is split into 3 clusters, as seen below. For the sake of example, let's say I want to find the differentially expressed genes in the uppermost ...
tensor's user avatar
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6 votes
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Is it possible to calculate p-values for fold changes of single replicate RNA-seq samples?

I'm thinking that this isn't actually possible, but I'd like to check before I write it off. I have RNA-seq data from cells under three different conditions, there are no replicates for any of the ...
J0HN_TIT0R's user avatar
7 votes
2 answers

Missing genes and normalisation of RSEM output using EBSeq

Without going into too much background, I just joined up with a lab as a bioinformatics intern while I'm completing my masters degree in the field. The lab has data from an RNA-seq they outsourced, ...
J0HN_TIT0R's user avatar