Questions tagged [exon]

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Downloading exon-wise gene sequence using commandline

I want to download exon-wise gene sequence of around 200 genes from NCBI. Is there anyway to do this from terminal? I have the transcript id for each sequence and I am using efetch command to download ...
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0answers
14 views

What is the tP statistic from ANGSD?

We were looking for genetic diversity in the exon level of an MHC transcript. We used ANGSD to calculate Thetas, Tajima and Neutrality tests and one of the thetas was a pairwise theta, however I am ...
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0answers
35 views

plot transcript expression vs length

I am working on long read RNA sequencing. I am interested in looking whether there is any bias on shorter gene against longer genes in my data as well as whether any bias towards 5' or 3' ends. Oneway ...
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38 views

Coding vs non-coding DNA length

I am trying to calculate the total exonic length (in bp) in order to see where the coding-noncoding ratio of roughly 1% to 99% comes from. We know all chromosomes total about 3 billion base pairs. So ...
6
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3answers
736 views

Can exons be located outside of the coding sequence?

I have a gff file like this (I edited the name): ...
3
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1answer
186 views

Pipeline for extracting gene from multiple genomes for use in HyPhy selection analyses?

I have been trying to obtain some preliminary data from HyPhy selection analyses to inform a larger project. I have obtained a number of assembled mammalian genomes from NCBI with the initial goal of ...
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2answers
56 views

Get gene exons from UCSC browser by HGNC name

I have a set of gene names in HGNC standard and I want to get all their exons (in BED format) from the UCSC browser but it seems like the browser doesn't index genes by their HGNC name. How can I do ...