Questions tagged [flow-cytometry]

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Simultaneously get data from multiple applied gates in flowCore

I'm applying two parallel and non-overlapping gates to a gatingSet directly under "root": ...
4
votes
1answer
67 views

What is the correct way of dealing with the analysis of data from flow cytometry?

I would like to detect a change in expression of a molecule present on a cell type by flow cytometry. Assuming I am able to detect, using an antibody, a signal that represents the amount of the ...
4
votes
1answer
585 views

What are negative FSC-A values in flow cytometry data?

I did flow cytometry analysis of E. coli cells 7 hours post inoculation (1% overnight culture in 3ml). I am analysing the FCS3.0 files using the flowCore package in R. I observe that FSC-A has ...
2
votes
1answer
78 views

Obtain CD (cluster of differentiation) marker expression information

There are 394 CD marker genes. Various resources (BD handbook, Abcam guide) contain information about which cell types express which CD markers. What is the best way to obtain or download this data so ...
2
votes
2answers
91 views

Finding peaks and estimating cell population sizes in multi-dimensional flow cytometry data

Our research institute processes a lot of flow cytometry data, but the produced data is under-utilised due to the effort required to process it. A typical run will produce 5 million events (ideally ...