Questions tagged [gene-ontology]
The Gene Ontology (GO) is a collection of annotations of genes from multiple species to terms describing the functional role of those genes. The terms are organized in a graph structure that allows for propagation of information. See http://geneontology.org
26
questions
0
votes
0answers
16 views
Retrieve gene symbols of genes from GO term with Python
Is there a way to retrieve the gene symbols belonging to a GO term with Python ?
For example, the GO:0006096 (glycolytic process) contains 96 genes:
...
0
votes
0answers
12 views
Functional similarity between 2 genes based on GO-tags
I need to evaluate the functional similarity or closeness of pairs of genes from Gene Ontology (GO) tags, and it is important that this similarity is not determined through sequence-similarity.
I ...
4
votes
1answer
43 views
How do I get GO annotations for a list of UniProt IDs?
I have a list of UniProt ids that I want to get Gene Ontology annotations for. I need this information because I want this high-level information as an input to a neural network. The model I wish to ...
0
votes
1answer
55 views
RDAVIDWebService (R package) manual installation, or alternative tool
Recently, I had to re-install all my R packages (R version 4.1.0 (2021-05-18) -- "Camp Pontanezen") and it turns out that "RDAVIDWebService" is ...
0
votes
0answers
19 views
How to identify specific motif for chip-seq data
For chip-seq data, I had analyzed the samples until motif discovery using both cluster profiler and Homer for each histone mark. I was wondering what is the efficient method to determine if each GO ...
0
votes
1answer
47 views
Retrieving all genes for a gene ontology term
I'm looking for an easy way to retrieve all the genes in a list that are associated with a certain GO term, preferably using R/Bioconductor packages. I'm not interested in under/overrepresentation or ...
2
votes
1answer
61 views
How to programmatically classify a protein according to its genbank feature
Say I found an interesting protein in a genbank file, e.g.:
...
0
votes
1answer
24 views
gprofiler output ENSG to gene name
I would like to ask if there is any tool or R script that translates Ensembl IDs to gene names in a gprofiler GO output. An example of this file is the following one:
0
votes
1answer
82 views
Parsing the json file from gene ontology result into dataframe
How do I parse the json file from ontology output.
My file json file
jdata <- read_json("analysis.json", simplifyVector = TRUE)
I want to convert it ...
0
votes
1answer
48 views
Bonferroni count = 1 in GO-Slim overrepresentation test (PANTHER) is it possible?
I encountered a problem while performing analysis using the PANTHER website. I run the statistical overrepresentation test for a genelist of 99 genes (GO-Slim BP, Binomial, Bonferroni) in February ...
1
vote
0answers
32 views
Gene Ontology equivalent - higher specificity?
I've recently discovered OrthoVenn2, which I'm using to compare proteomes and extract likely clade-specific genes. I'm enjoying software, and one of the things it offers is a breakdown of protein ...
2
votes
1answer
58 views
Analyzing proteins based on sequence similarity
Using NCBI Gene database, I retrieved list of 7015 protein which are implicated in autism pathogenesis. This list includes tax-ID, organism name, symbol, aliases, map location, chromosome, genomic ...
1
vote
1answer
67 views
How to identify latent variables in single-cell RNA-Seq data
I have a single-cell RNASeq sample, in which I'd like to identify latent variables (e.g. response to stress) that I think might be affecting the clustering.
The approach I was planning to use is to ...
0
votes
1answer
271 views
Interpreting a kernel density function plot of gene expression
I need help interpreting a kernal density plot and a histogram of this data. I have provided some context below let me know if you need more information
A co-worker that I am collaborating with has a ...
0
votes
1answer
36 views
Why are my genes filtered for Gene Ontology term enrichment?
When I enter a list of gene names into common gene ontology (GO) term enricher (e.g. MsigDB or ToppGene) only a small fraction of genes is actually used for the enrichment:
I am struggling to ...
1
vote
0answers
24 views
Gene Ontology Specific Fold Changes
I am currently doing a project involving gene rank correlations. How we rank our genes is based on the absolute values of the log2 fold change. I.e. a gene which is doubled with respect to the ...
2
votes
1answer
86 views
Discordance of Gene Ontology data between Mouse Genome Informatics and Ensembl sources
In my first approach to the use of Gene Ontology, I have found a discordance if we consult either Mouse Genome Informatics (MGI) or Ensembl for these data.
Concretely, for the mouse genes Zic1 (NCBI ...
0
votes
2answers
31 views
How do i map subcellular localizations to lower the number of classes to predict
For a project, I want to predict the subcellular localization of some proteins. I am a student in software Informatics at the University of applied Science HEIG-VD.
Subcellular localization ...
0
votes
1answer
117 views
How do I perform a pathway related grouping of genes?
I have a list of gene names from the analysis of my mouse single seq data. I want to perform a simple grouping of the genes based on the pathways to which they belong or pathways through which they ...
3
votes
1answer
141 views
different results coming from biomart online and biomaRt R library
I am playing with biomart in order to get the list of genes associated to a specific gene ontology (GO) term.
So, if I use the webservice as in this query:
I get at least 50 genes. If I download the ...
0
votes
1answer
684 views
Retrieve all genes for a specific gene ontology
I'm having a bit of confusion about how can I retrieve all the genes that are associated to a specific gene ontology.
Lets say I need to find all the genes that are associated with this GO entry:
...
0
votes
1answer
38 views
NCBI xml-format Printing nodeValues from tags with the same names
I would like to retrieve all Other-source_anchor on the level of DNA binding from within a Gene Ontology XML file using php.
If I try this with my code, I only get Gene Ontology back and not the ...
1
vote
0answers
75 views
How can I create my own GO slim?
I know there are existing GO slims predefined. But does anyone know how I can create my own collection of GO terms for enrichment analysis?
2
votes
1answer
77 views
Obtain CD (cluster of differentiation) marker expression information
There are 394 CD marker genes. Various resources (BD handbook, Abcam guide) contain information about which cell types express which CD markers. What is the best way to obtain or download this data so ...
4
votes
2answers
188 views
Retrieving a list of human genes having GO associations
I need a command in R to retrieve all human genes associated with a Gene Ontology entry. I tried to look for it online but did not find it.
7
votes
1answer
871 views
How can I create my own GO association file (gaf)?
This question is based on a question on BioStars posted >2 years ago by user jack.
It describes a very frequent problem of generating GO annotations for non-model organisms. While it is based on ...
