Questions tagged [gene]

Use this tag to refer to the gene sequence/coding part of the sequence.

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12
votes
6answers
1k views

Converting gene names from one public database format to another

This is a question from /u/apivan19 on reddit. The original post can be found here. I have some proteomics data that was given to me with the UniProt gene identifiers in column 1. I've been trying to ...
11
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1answer
873 views

How to read and interpret a gene expression quantification file?

I have a gene expression quantification file from TCGA that contains the following lines: ...
11
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2answers
4k views

How can I calculate gene_length for RPKM calculation from counts data?

I have read counts data and I want to convert them into RPKM values. For this conversion I need the gene length. Does the gene length need to be calculated based on the sum of coding exonic lengths? ...
10
votes
4answers
737 views

How can I build a protein network pathway from a gene expression quantification file?

Assume I have found the top 0.01% most frequent genes from a gene expression file. Let's say, these are 10 genes and I want to study the protein protein interactions, the protein network and pathway....
9
votes
3answers
2k views

How to identify gene expression signatures from gene expression data?

I have TCGA gene expression data. I'm interested in identifying gene expression signatures using the data. I would like to know whether there are any tools or R packages for identifying gene ...
8
votes
2answers
58 views

Melt p-values for CpG sites mapping to the same gene

I have some data I am working with, and I am curious if I am able to combine p-values from a paired t-test for CpG sites in the genome using Fisher's Method to get one p-value for each unique gene. ...
8
votes
1answer
451 views

Comparison of gene set enrichment statistics

I am performing a gene set enrichment analysis to determine if particular gene sets are coherently up- or down-regulated. I have seen several statistics for computing a p-value of GSEA-style ...
8
votes
1answer
296 views

What's a good ontology for drug names?

I have ... A database with patient phenotypes in, stored as HPO terms Genetic data in whatever format I need I want ... To store drug names in a way that won't make my life difficult If it's ...
7
votes
3answers
250 views

How to detect a mutation and predict its consequence?

I read lecture notes about mutations, and wondered what kind of algorithms are there to detect mutations? How do we know if the gene is mutated or whether it is a sequencing error? I saw this thread ...
7
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3answers
3k views

Retrieve detailed gene descriptions

Given a list of gene IDs, how do you retrieve the gene description, summary and other detailed information in R?
7
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2answers
127 views

Are gene names same across species?

I have a bunch of gene names of Apis mellifera (specifically 194). I used these gene names as an input on STRING database to create a network for Drosophila melanogaster. 54 of those genes were also ...
7
votes
2answers
9k views

Converting Ensembl Gene IDs to Entrez Gene IDs through biomart

Well, I'm trying to convert a list of Human Gene referenced by Ensembl Gene IDs to Entrez Gene IDs. I have been advised to use biomart. I tried to get a kind of conversion table for all human genes. ...
7
votes
1answer
228 views

What is the etymology of "Entrez ID"?

Since I have seen NCBI gene names were called "Entrez ID" for the first time, I am wondering where that comes from. Such a weird name! Does anybody know where that originates? My hypothesis is: in ...
6
votes
3answers
714 views

Can exons be located outside of the coding sequence?

I have a gff file like this (I edited the name): ...
6
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3answers
300 views

Fast filtering of intervals not falling within a certain distance from known genes

I would like to filter a bed file with intervals, ie. in the format of: chr1 13800 14301 chr1 15500 16001 chr1 19400 19901 chr1 22800 23301 ...
6
votes
4answers
473 views

A good tool for gene locus visualization

Does anyone know of a good tool for visualizing gene loci? Basically, I'd like to show how a group of genes look in different strains of a bacteria. I have annotation of course, I just need a tool to ...
6
votes
3answers
72 views

Do any publicly available databases detail protein structure and functional domains?

I am interested in finding a database that takes a gene or protein name as input (possibly with the option to specify transcript) and gives information about the protein's functional domains in terms ...
6
votes
4answers
2k views

Finding gene length using ensembl ID

I want to find the length of a list of genes, of Homo sapiens, that is reported in the GEO database. I have gathered the ensembl id's of those genes. I understand this information can be parsed from ...
6
votes
2answers
448 views

How to isolate genes from whole genomes for phylogenetic tree analysis?

I have 446 whole Klebsiella Pneumoniae genomes I want to build a phylogenetic tree from. After reading about constructing phylogenetic trees it seems the only option for large numbers of genomes is to ...
6
votes
3answers
139 views

How to find GEO data sets using Drug Bank ID and bioDBnet

I want to find all experiments in GEO that are associated with a drug (for example tolvaptan). Is there any quick and scalable way to to this? I want to query more than 100 drugs. I tried to use ...
6
votes
1answer
888 views

Entrez or Ensembl gene IDs?

I'm using several datasets that are encoded using either Entrez or Ensembl IDs to specify genes, and need to decide on which to standardise on. Are there any major reasons to use one over the other? ...
5
votes
6answers
4k views

How to get a list of genes corresponding to the list of SNPs (rs ids)?

Is there a way for me to get a list of genes given a list of SNP rs ids? I found several questions asked with a similar goal years ago, and the answers are always about using multiple online tools, ...
5
votes
3answers
186 views

Where can I find a database that has phenotype information together with associated SNPs?

I have been searching for a long time and the furthest I got is some database with the functional description for genes. Then, I have to parse these descriptions manually to figure out the association....
5
votes
1answer
133 views

SNP-phenotype association analysis only using the SNPs on a specific gene

I know that the GWAS association p-value threshold is 1e-8. This makes sense because in GWAS, you make ~1 million hypothesis (i.e. use that many SNPs in the association test). However, let's say I do ...
5
votes
1answer
558 views

How to get results from Homo.sapiens package in bioconductor for a specific reference

I want to use the Homo.sapiens package in Bioconductor to retrieve the chromosome location start and end for each gene symbol in a specific reference (e.g. hg19 or hg38). Right now, I am using the ...
4
votes
3answers
216 views

What are the different kinds of bioluminescent genes?

I know of the common green glow gene but I forgot the name and I also know that some algae glow blue. There are so many types of bioluminesent organisms, so I am wondering what species have which ...
4
votes
2answers
142 views

Writing a perl script to holding information for two genes

Basically I have a perl script in which I have an array (where each element of the array references a hash) and need to be able to print the array with a dumper function. Thus I need to be able to ...
4
votes
4answers
138 views

What are the standard ways to visualize protein-protein or gene-gene interactions?

I would like to visualize this interaction list. Is there an online/web based way to do it? Is there a way to analyze the data? Assuming I have exported the list into lines such as: ...
4
votes
1answer
182 views

Duplicate gene symbol handling in GEO gene expression data

I have downloaded a gene expression data from GEO database (GSE3268) in which in some of its rows there are duplicate gene symbols. For example TP53 exists in two rows with different expression values ...
4
votes
3answers
367 views

Expression of a gene in different groups

I would like to check the expression of a gene in different groups like Disease vs Normal samples. I want to make a plot out of that to check whether it is significant or not. From this paper lncRNA ...
4
votes
1answer
863 views

Inspection of gene expression in scRNA-seq data

I am running the data preprocessing pipeline for scRNA-seq data presented here. 3.8.6.1 Gene expression In addition to removing cells with poor quality, it is usually a good idea to exclude genes ...
4
votes
0answers
56 views

Correcting for noise in RT-qPCR gene expression data

I have a training set of RT-qPCR gene expression data (not run in triplicate) for a batch of samples with two phenotypes $A$ and $B$ on which I've trained a "logistic regression classifier". ...
3
votes
4answers
582 views

Basic questions about GSEA

I'm currently learning about Gene Set Enrichment Analysis (GSEA) in the hopes of using it in my analysis of differentially expressed genes, and I just had a few questions about the program, ...
3
votes
2answers
194 views

Why are some of the gene peptides returned by biomaRt missing an asterisk in the end?

Note: this question has also been asked on BioStars I am getting peptides using biomaRt library in R for the gene 'BRCA1'. 27 different aminoacid sequences are returned, and 12 of those have an ...
3
votes
2answers
20k views

Converting Gene Symbol to Ensembl ID in R

I'm trying to convert ~20,000 different human gene symbols to ensembl IDs. I've been trying to use biomaRt to do this, but continue getting the following error ...
3
votes
2answers
67 views

Identifying relevant SNPs from a list

I have a list of all SNPs (a list of RsIDs) that fall in the coding sequence of several thousand human genes, and I'm looking for a convenient method to come up with a list of known SNPs that alter an ...
3
votes
1answer
846 views

Missing data mappings in mygene.info while trying to convert Genes Ensembl Ids to Entrez Ids

I need to convert a lot of Ensembl Ids to the relative counterpart in Entrez (e.g., ENSG00000157764 > 673). I found mygene.info ...
3
votes
2answers
57 views

Associating SNP and GENE

Assuming I have SNPs data using hg19, how can I know which SNP belongs into which Gene? The data looks like: chr10_103577643 chr10_124712463 and so on. I ...
3
votes
1answer
222 views

What are the coordinates of the extended major histocompatibility complex (xMHC) in GRCh38?

Is there an accepted boundary of the extended major histocompatibility complex (xMHC) in human? Specifically, I am interested in the boundary coordinates for Genome Reference Consortium Human Build 38 ...
3
votes
2answers
59 views

Where can I find gene expression data on one of the cell lines in NCI-60?

I'm trying to retrieve the most expressed genes from a cell line from the NCI-60, lets say cell line NCI-H23, but I'm not sure where to find the gene expression data. My main goal is to create a ...
3
votes
2answers
284 views

How can I obtain a list of all NCBI gene ID's along with their full name, symbol, and also known as symbols?

I would like to do two things: (1) Obtain a list of all NCBI (Entrez) gene ID's. (2) Obtain the "Official Symbol", "Official Full Name", and "Also known as" fields from ...
3
votes
2answers
544 views

How can I find the chromosomal location of a list of genes?

I have a list of genes nearly 20000: gene name (column1) and coordinates (columns 2 and 3) ...
3
votes
1answer
181 views

How to download gene expression data from NCBI gene database

In the NCBI gene database, I can add the expression tracks (circled in picture blow) through 'Tracks' button, but How I can download the expression data directly, not just look the picture?
3
votes
1answer
50 views

How to read gene regulatory network edge list files?

The following is an excerpt from an edge list file from the Gene Regulatory Database, YeastNet v3 ...
3
votes
2answers
139 views

Hemoglobin subunits genes in scRNA-seq

In one scRNA-seq sample I encountered the genes: Hbb-bs, Hba-a1 and Hba-a2. These genes appear on top of the list of the highest expressed genes but the 75% percentile of cells have very low counts. ...
3
votes
1answer
34 views

Is there a way to tell which chromosome a gene is on, by looking at the "Chromosome/scaffold name"

I recently got a data set, from which I need to figure out which chromosome a gene is from, but the head of the data reads like: ...
3
votes
1answer
128 views

Combine multiple species trees

I have five species trees run using starBeast and Beauti. Here is a link to the paper discussing both, http://journals.plos.org/ploscompbiol/article/file?type=supplementary&id=info:doi/10.1371/...
3
votes
1answer
39 views

How does Cellminer's "Cross-correlations of transcripts, drugs, and microRNAs" work

I have seen one of Cellminer tools. I am not sure how do they calculate the cross correlation of the genes, what does it actually mean? Based on what databases? For example if I take their example ...
2
votes
4answers
89 views

Separate multiple sequence into different file, one sequence per file

I have a file contain multiple sequence, and I want to separate them by "gene:" into different file. example: example.fa ...
2
votes
2answers
70 views

Using data mining of papers in order to derive genomic connections [closed]

Are there any examples of researchers data mining articles and papers (e.g., from pubmed or google scholar) in order to derive relations between genes and diseases?