Questions tagged [genomics]
Genomics is an interdisciplinary field of science focusing on the structure, function, evolution, mapping, and editing of genomes.
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20 views
NMF - Subscript out of bounds
I've calculated the cophenetic coefficients for the NMF of gene expression data, but is giving error when performing the clustering information step as given below:
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1answer
51 views
Difference between bioinformatics and cheminformatics [closed]
Anyone can answer, and should be in simple way
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1answer
32 views
Statistical or performance test for genomic intervals between 2 samples
Is there an appropriate statistical or performance test to perform when I want to compare genomic intervals between 2 samples. Sample A is the the benchmark of comparing the intervals and sample B is ...
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0answers
10 views
How can I create a no-frills view config file for HiGlass?
Is there a basic example anywhere that explains how to create a no-frills view
config to view the contact map for a non-human genome assembly using the
server-based viewer at http://higlass.io?
I ...
0
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1answer
38 views
NMF clustering not giving results
I'm trying to run the Non-Negative Matrix Factorization(NMF) for my Gene Expression dataset which was originally in matrix form. But it throws errors as given below:
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1answer
17 views
Transfer annotations from one genome assembly to another
I've got an annotated draft genome assembly made using short and long read strategies. I've also done optical mapping to stitch some of the contigs together, with the goal being chromsome-length ...
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1answer
26 views
Mapping unique GO term description given a specific GO ids
I have a list of GO ids and I want to find out a unique term description such that if I provide say 200 GO IDs I will give 200 specific GO terms. The code snippet I am using is given below. Although ...
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2answers
44 views
Counting the number of gene isoforms in a GFF3, is this method correct?
Recently I've been tasked to count the numbers of gene isoforms for each locus in a .gff3 file. I'm still doing my first steps in biology and bioinformatics, so I ...
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0answers
13 views
General Question: Statistical Alternatives to Oncoprint
I am a computational genomics student who has recently joined a research lab as an RA. We often work with oncoprints (comuts/heatmaps), and we visualize molecular signatures by reorganizing the comut. ...
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1answer
30 views
Parallelizing microRNA targets
I am trying to look for miRNA targets using a file called Conservedfamily.txt from the Zebrafish target scan fish website. I have written a python program to ...
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1answer
74 views
How to download data from SRA in Linux systems via the command line?
My workflow for downloading data from SRA has been the following:
Access SRA Run Selector.
Enter the accession number for the project of interest.
Download "Accession List" for the "...
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0answers
38 views
How to modify dot plot in MUMmer 3 for bacteria comparative genomics?
I was trying to make a dotplot to visualize genome-genome sequence alignment by using the MUMmer 3 software (it is typically used to compare whole genome sequences of bacteria). I runned the same ...
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32 views
Difference in BLAST and eggNOG
Note: this question has also been asked on Biostars
This is question regarding the tools rather than the concepts.
I am a freshman at college and I am a working on an evolutionary biology of ...
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0answers
58 views
Conversion of SAM to BAM files
I am very new to micro RNA analysis. I have been using H. sapiens, GRCh38 + major index as given in the Bowtie Website to align with my trimmed FASTQ file .
The command I am using to make very ...
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0answers
16 views
Average ratio of spliced and unspliced mRNA in human scRNA-seq data
I am currently doing RNA Velocity Analysis using scRNA-seq data from human blood and skin. The RNA Velocity is calculated using the balance of spliced and unspliced mRNA. One thing my PI and me were ...
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0answers
37 views
using Bowtie to map miRNA-seq data to rfam and also reference genome
I am using Bowtie to remove non-coding RNAs (tRNA, snRNA, rRNA) by rfam and also maping our microRNA-seq data with mirbase using following code:
...
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1answer
43 views
RNA_Seq data aligned used uniquely or multi mapped reads impact on result interpretation
I have some transcriptomic (Whole) sequencing data that I should analyse. I would like to do raw data alignment to a reference genome taking into account the multi mapped reads and uniquely mapped ...
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0answers
8 views
Barrett's esophagus databases and web tools
I would like to ask if anybody knows public domain databases and web tools to analyse different stages of Barrett's esophagus. The most that I can find are esophageal cancer databases with patient's ...
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1answer
17 views
How do you find differences when your metagenome sample size is small?
I received a matrix of genomics features (KEGG annotated metagenomes) abundance from 6 samples belonging to 2 groups. My collaborator is interested in finding differences between the two groups.
The ...
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1answer
54 views
Converting FASTA Sequences to SNP FASTA Format
I have two FASTA sequences. Each is from a different species' mitochondrial genome. The sequences are pasted below.
...
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1answer
27 views
how to get access to genotypes and phenotypes used for a GWAS
I'm a master's student working on genomic prediction of complex traits using deep learning.
i'm looking for a dataset of human genotypes and phenotypes that has been used for a GWAS.
The only thing i ...
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2answers
55 views
Finding SNP/Indel Markers of Interspecific Variation
This is a revised version of my original question, edited to include the portions already answered by @Maximilian Press.
Goal:
Find SNP and indel alleles which are fixed between but lack polymorphism ...
1
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1answer
94 views
How do I create a VCF file of all known pathogenic mutations in a gene of interest?
I would like to create a list (probably .vcf format would be good) of all known pathogenic missense mutations in a human gene of interest and then add other variants that could lead to the same ...
0
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1answer
52 views
Nvidia Parabrick fq2bam pipeline error - No such file or directory
I am trying to implement Nvidia-parabrick fq2bam pipeline to process my WES dataset. The reason for opting this pipeline is the huge number of samples (1004) which take many months to process for ...
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1answer
56 views
How to count RNA sequencereads using custom made python scripts?
I am trying to do RNA seq analysis and my goal is to filter gene counts less than 5 using custom made python scripts. The code chunk goes as follows
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0answers
33 views
Genomic relationship matrix explanation
I am seeking the definition of the genetic distance matrices used in genomic prediction.
What is the difference between a Rodger distance and kinship matrix?
what people mean when they talk about ...
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0answers
25 views
make recombination genetic map
Given a Plink genetic map with 4 columns: Chromosome, rs-snp, Map(cM), Position(bp) and approximately 8,000 lines find the recombination rate [Rate(cM/Mb)] from a HapMapII genetic map of 4 columns: ...
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56 views
How to extract certain genes including non-coding regions?
I would like to look at the non-coding regions of some genes. I have around 1000 full genome assemblies and I was able to extract the nucleotide sequences for certain proteins with ...
2
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0answers
17 views
When to use Ariba vs Roary or other genomics tools to call drug-resistance variants?
I have a large dataset of assemblies of bacterial S. aureus genomes (~1000, same species). I am looking at different tools to estimate the presence of drug-resistance factors such as blaZ or ampC. ...
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1answer
248 views
What are the coordinates of the extended major histocompatibility complex (xMHC) in GRCh38?
Is there an accepted boundary of the extended major histocompatibility complex (xMHC) in human? Specifically, I am interested in the boundary coordinates for Genome Reference Consortium Human Build 38 ...
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1answer
36 views
How to find Genotype, from list of mutations:lineage list?
I'm very new to python and I'm getting along nicely (I'd like to believe); however, I must be missing something here. I'm looking to read each and every file and compare each line against one another.
...
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1answer
65 views
How to get % similarity between strains and mutation files
I’m very new to python, and having some difficulty getting hang of some more complicated things
I have multiple files which look like so:
hCoV-19/Singapore/4/2020|EPI_ISL_410535|2020-02-03
hCoV-19/...
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1answer
58 views
How to identify unknow bacterium species from whole genome genetic sequence
I am a Biochemist that is unfamiliar with bioinformatic tools and new to academia as a whole.
I am currently using ILLUMINA PE data, which I trimmed (Trimmomatic), corrected (Rcorrector) and assembled ...
0
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1answer
62 views
Pointing Cromwell to local EBS mounted storage on AWS Batch
I'm trying to run the WDL tasks from the GTEx RNA-Seq pipeline with Cromwell using AWS Batch as a backend.
I store the STAR alignment index on an Elastic Block Store since my Cromwell server instance ...
2
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1answer
91 views
COVID-19 GWAS: what is a cross-replicating association?
In a genomic study of patients infected with SARS-CoV-2, the authors detected cross-replicating associations with rs11385942 at locus 3p21.31 , the association signal spanned the genes SLC6A20, ...
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1answer
39 views
How to annotate gene length to a list of gene symbols using UCSC data?
I have a list of HGNC gene symbols, I am looking to get the gene length of each gene. Although I also describe these genes with lots of UCSC datasets as features, so I am wondering if there is a ...
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0answers
36 views
"How the clustal omega can be reverse engineered, to trace ancestral inversion mutations via the guide tree?"
I apologise for very basic question but I am very new to biology and have very specific knowledge of this field, I am currently working on Bioinformatics in my machine learning project which is why I ...
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1answer
63 views
How to compact variant data to their genes without bias?
I have a dataset of genes I am trying to collect data on from public databases, to use as features in machine learning. I am trying to take some features from UCSC genome browser (e.g. number of CpG ...
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2answers
63 views
How identifiable are human omics data and how to mitigate their identifying features?
Say a database were to store human omics datasets. The human subjects are known and the sample size is rather small in size initially (n=500). The database contains genomics, transcriptomics, ...
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0answers
251 views
Kraken2 > OTU format > Phyloseq
A collaborator has passed me over Kraken2 outputs *.report and *.kraken, from a metatranscriptomic sequencing experiment conducted on the minION.
I would like to use this data in phyloseq, however, I ...
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1answer
31 views
What is the origin of the 'source Gene ID' references given in the 'gene_presence_absence.csv' output of Roary?
I am learning to use Roary for preparing a pan genome for some lactobacillus strains. In the 'gene_presence_absence.csv' output of Roary (which I view in excel), a 'source Gene ID' is given for each ...
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29 views
How do I delete ligands from a list of 50,000
I need to delete ligands from a list of over 50,000 ligands in a .sdf file format. Is there any easy and quick way of deleting the ligands from the list.
Thank you,
Dr. Hussain.
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0answers
51 views
How to aggregate multiple MAF files together?
I have cancer genomic data (tumor/normal whole exome sequencing) from 50
patients that received the same type of treatment, half of whom responded. These come in the form of 50 .maf files, along with ...
0
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1answer
19 views
Is the number of contigs and scaffolds an artifact of genome assembly and rather arbitrary, or is it canonical for each species?
E.g. bowhead whale genome has X contigs and Y scaffolds, but couldn't these easily be different numbers?
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2answers
102 views
How to combine two Genome-wide Association Study (GWAS)? [closed]
I did a GWAS analysis in the past for antibiotic resistance of E. Coli and the results were interesting (matching the literature). I did a new GWAS analysis for some new samples, but the results are ...
1
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1answer
87 views
Problem with classification model of genomic data: every machine learing model predicts wrongly almost always the same subset of dataset
First of all, I'd like to apologize for any spelling or grammar mistakes.
I'm having a problem using R for a classification problem. My dataset contains ~300.000 genomic data, and the features are ...
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1answer
38 views
Phasing partially phased genomic data
I am trying to create an integrated callset with coding regions from phased exomes and noncoding regions from a genotyping array. I would like to merge the data together in the correct phase. Since ...
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1answer
43 views
How to compute Shanon entropy
I want to analyze some viral sequences. I want to use shanon entropy as a measure to analyze the sequences. However I do not understand the concept of shannon entropy. Would anyone explain please how ...
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0answers
28 views
What Cellbase resources are 0-indexed, and which are 1-indexed?
The UCSC Genome Browser Team is clear about that the genome browser GUI indexing which is 1-based, closed interval. Genomic sequence retrieval via the UCSC REST API is 1-based, closed interval. All ...
