Questions tagged [long-reads]

Questions relating to long reads, such as pacbio or nanopore reads.

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Platanus-allee phasing fail: Error(13): Error, SolveDBG exception!

I am using Platanus-allee 2.2.2 for heterozygous genome (~500mb) assembly with Illumina short reads and PacBio reads input data. I have the file contigs.fa from short reads but phasing step with ...
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Why does samtools mpileup sometimes include ref bases (other than ',' or '.')?

This is my first post here. I can think of no way of giving an easily reproducible example, as per the stack ethos. SO apologies in advance and any feedback on question format appreciated. I have ...
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plot transcript expression vs length

I am working on long read RNA sequencing. I am interested in looking whether there is any bias on shorter gene against longer genes in my data as well as whether any bias towards 5' or 3' ends. Oneway ...