Questions tagged [mass-spectrometry]
The mass-spectrometry tag has no usage guidance.
11
questions
3
votes
1answer
26 views
Bruker MALDI-TOF bacteria species identification scoring algorithm
Just wondering whether anyone can point me to a research paper which describes how the scoring values are generated by the Bruker software for species identification of bacteria in MALDI-TOF MS. For ...
1
vote
1answer
17 views
Why don't the “child” peptide masses sum to the parent in this De novo sequencing example?
In that example, 129+216+327 does not equal the parent's mass of 492.
My understanding is that during MS, the protein is broken down into peptide masses of varying length of approx. 5 to 20 amino ...
1
vote
0answers
26 views
Hybrid approach in metabolomics (NMR and MS)
Is it currently common in metabolomics to combine two quantification methods, namely NMR (nuclear magnetic resonance) and MS (mass spectrometry, either liquid or gas)? If so, what would be profits ...
1
vote
2answers
806 views
Running MaxQuant on Linux
I'm trying to run MaxQuant on a linux laptop, hower I'm constantly running into problems with MaxQuant crashing at different stages
I tried to use
mono 6.8
mono 5.4.1
on
centos
ubuntu
each with ...
1
vote
1answer
15 views
Can Maven (mass spectrometry software) read mzML files?
I did not find a documentation. Can Maven (mass spectrometry software) read mzML files?
2
votes
1answer
57 views
Principle of TMT Tags in Multiplex Proteomics
I am new to proteomics research and analyzing mass-spec data. I am trying to learn more about the theory/principle of TMT tagging (a type of isobaric labeling) coupled with LC-MS/MS spectrometry. I ...
1
vote
0answers
94 views
How to do protein quantification with Peptideshaker output (TMT labeled, MS2)?
I have TMT tagged MS2 mass spec run processed with Peptideshaker. I saved the project in a peptideshaker format cpsx. Now, I would like to do the quantification ...
1
vote
0answers
36 views
How to generate mgf files from Orbitrap generated MS2 proteomics raw files?
I tried to use RawTools to generate MGF files containing peaklists extracted from MS2 mass spectrometry raw-files from an Thermofisher Orbitrap. The command I used was
...
0
votes
2answers
156 views
How to run proteowizzard on linux using wine?
I am trying to run proteowizzard using wine to be able to convert raw mass spectrometry files to myML format. Following, these instructions` which seem terribly outdated. I am getting many issues. I ...
5
votes
2answers
2k views
How to run MaxQuant in command line mode?
MaxQuant is a software package for mass spectroscopy and proteomics. There is a windows version and a linux version. To run on linux you have to use a program that is called ...
1
vote
0answers
20 views
XCMS isn't evaluating the proper peak due to retention time drift
I'd like to do a targeted screening analysis for trace contaminates. However, due to the nature of the sample preparations, the retention times I'm getting drift a lot from run to run and XCMS isn't ...
