Questions tagged [motifs]
Physically linked short region of DNA or most notably amino acids which characterise a 'pattern' identifyable by optical analysis.
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How to identify specific motif for chip-seq data
For chip-seq data, I had analyzed the samples until motif discovery using both cluster profiler and Homer for each histone mark. I was wondering what is the efficient method to determine if each GO ...
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1answer
23 views
How to get matching pattern along with ID in a single command in grep?
I have a file containing multiple fast sequneces. For a specific consensus pattern as input, I extracted all the matching patterns from target fasta sequences with
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2answers
48 views
biopython-motifs , How can I create motif from sequences of different length?
I want to create motif of different length kmers.
Following code works for kmers of same length but not different length.What to do ?
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3answers
137 views
Finding motifs: fasta file with 10,000 sequences
I am new to Python. I am trying to parse a fasta file containing 10,000 sequences to look for motifs (microsatellites in particular). I tried using Seq Utils to parse my sequences for a particular ...
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Greedy Motif Search [ Reasoning ]
I was able to execute the algorithm correctly. But I'm still trying to get a hold of the result I'm achieving.
This is the algorithm I'm trying to understand -
http://rosalind.info/problems/ba2d/
If ...
3
votes
1answer
101 views
Human Herpes Virus, fusion motif in protein vs. SARS-CoV-2?
Is it possible that the fusion with cell propriety of Human Herpes virus, I see X14112.1 in GenBank is due to protein UL36 (tegument of virus) starting in linear position 2685 ???
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32 views
MEME's motifs have an E-vaue too high
I'm currently learning how to use MEME-Suite (web tool) to find a specific known transcriptor binding site in the promoter region of 5 putative bacterial genes of interest, but the results obtained ...
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0answers
42 views
Profile HMM to find sequence motif using Baum-Welch instead of using a given multiple sequence alignment?
I know this can be very problem-specific -- but it seemed to me the standard approach to train an HMM to recognize a binding pattern of a protein is to use the multiple sequence alignment (MSA), ...
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2answers
3k views
Python - Finding a motif - input: a txt file with 10 sequences and 10 motifs
When I run my BruteForce function with only one input it works and the result is correct.
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1answer
58 views
De novo motif discovery in protein sequences
I am trying to build a features matrix to be used for Random Forest based classification. I'd like to add, as features, short motifs which are common to all the protein sequences belonging to a ...
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0answers
16 views
Finding gene name from human genome using SP1 transcrition factor binding site from Postion Weight Matrix
I have a list binding site motif of SP1 transcription factor which collects from PWMScan tools. Actually this tool scan whole human genome and give the entire binding motif list. From that list of ...
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1answer
97 views
Motif Discovery in Bipartite networks using Cytoscape
I am trying to find over-expressed motifs in a bipartite transcription factor gene network.My genes are not very well characterized, so finding functional motifs has been a challenge. I used the motif-...
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1answer
1k views
Biopython Phylogenetic Tree replace branch tip labels by sequence logos
Having recently constructed a lot of phylogenetic trees with the module TreeConstruction from Phylo package from Biopython, I've been asked to replace the branch tip labels by the corresponding ...
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1answer
117 views
Creating frequency plot from weblogo with frequency unit on the Y axis
Hello I am trying to create frequency plot for a motif with frequency unit on the Y axis from 0 -100 nt as the unit as represented in this paper.(Seiler et al., 2018)
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1answer
126 views
Searching for protein motifs with MEME
I am trying to look for motifs in a set of proteins returned by a mass spec experiment using MEME. The experiment was carried out in the yeast Candida albicans.
Does anyone know if it is worth ...
3
votes
1answer
170 views
Database of position weight matrices for protein motifs?
I am trying to identify proteins that carry a consensus target sequence for a kinase. Usually if I am working with, for e.g. transcription factors binding to DNA I would use position weight matrices (...
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1answer
925 views
Where to download JASPAR TFBS motif bed file?
I am interested in determining if any transcription factor binding site motifs are enriched in some BED files from a DNA methylation experiment.
I am looking for a database that has BED Files ...
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2answers
632 views
Where are .motif files from homer knownResults?
I have been using homer's findMotifsGenome.pl, but with my new version (v4.9.1) of homer I don't get ...
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votes
5answers
439 views
What motif finding software is available for multiple sequences ~10Kb?
I have around ~3,000 short sequences of approximately ~10Kb long. What are the best ways to find the motifs among all of these sequences? Is there a certain software/method recommended?
There are ...
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2answers
241 views
How to calculate statistical significance of sequence motifs
I would like to show that certain types of RBP motifs are enriched in RNA editing islands (i.e. clusters of RNA editing). However, I am unsure about how to think about sequence motifs with respect to ...
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3answers
136 views
Have DNA motifs 6-12bp long, trying to get conservation scores
I have about 200 short nucleotide motifs (6-12 bp in length) from the human genome, and I'm trying to see how conserved they are across vertebrates.
I was thinking that I'd need to make a bed file ...
