Questions tagged [multiple-sequence-alignment]
The multiple-sequence-alignment tag has no usage guidance.
10
questions with no upvoted or accepted answers
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How to get phylogenetic tree from multiple genes?
I constructed a phylogenetic tree using a gene (example - secA). I had to gather the same gene sequence for all the required species from public database-NCBI and then constructed the tree after ...
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2
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Alignment with inserts and keeping the indexing of ref seq intact
Parts of sequences are given below-
Reference sequence (pre-alignment):
ATTAAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGT
...
- 145
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Why do I obtain different output results with blast vs awk commands
I have an awk command that identifies 30 pb from two multifasta files.
When I used two input files:
E.g. 100 sequences each, I get the same result with the ...
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1
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36
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Aligning sequences with multiple genetic codes!
I am doing a project on duplicated genes and I have a major difficulty on how to align sequences that use different genetic codes. I work with fasta files that contain sequences of protein coding ...
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SNP How to detect regions of erratic pairwise alignment?
I have clusters of DNA sequences that contain some mutations. All the sequences in a cluster should contain the same mutations.
As the mutations aren't known, those clusters of sequences were pairwise-...
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difficult in runing the R code for ggtree
I tried to run the below code but ended having issue. I have checked thoroughly and I didn't find any mismatch in fasta sequence
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29
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Call NCBI Multiple Sequence Alignment Viewer with target URL?
I am storing a sequence aligment file on a webserver and now would like to visualize the results. I used the NCBI Multiple Sequence Alignment Viewer . The issue is that there are several manual steps ...
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Mapping List of Probes/Primers/Short Oligos to a Reference Fasta/q
Could you help me bioinformatics SE people.
So I have been duckduckgo-ing up a storm and even consulted the AI overlords and haven't come up with a solid way to do this, but it is so fundamental to ...
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Fastest way to count where two bam files are homozygote reference (inverted variant calling)
Maybe I'm having a brain-freeze so excuse me if this is a waste of everyone's time...
I am working with an organism with ~700Mb genome.
I have bam files of two individuals that are whole genome ...
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Splitting .bam files into separate samples for tophat2
I have RNA seq data of 12 samples (paired end, so 24 files) and I ran tophat2 for alignment on all of them which gave me one bam file for all the samples. Now I need separate bam files for all the ...
