Questions tagged [psi-blast]

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should I continue the iteration of psiblast if the blast step produced only below threshold sequences?

I used a PSIBLAST (position specific scoring) to detect the similarities between specific proteins and the database of Clostridium. The results did not reveal any similarities above the e value ...
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1answer
71 views

Error in PSI-BLAST when running psipred locally

I am very new to bioinformatics and Linux and am trying to run psipred locally on Ubuntu 18.04 but cannot get PSI-BLAST to work. I have downloaded BLAST legacy version 2.2.26 and unpacked it, uniref90 ...
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1answer
381 views

How to get PSSM of protein by protein sequence with python? [closed]

I didn't find way that convert protein sequences in FASTA to PSSM by python, and how to write program in python to find PSSM for our data set ? Maybe better to question how to find PSSM from NCBI ...
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1answer
416 views

How to interpret a PSSM matrix from PSI-Blast - what do the numbers represent?

PSI-Blast on a sequence returns a PSSM matrix file containing two matrices, one to the left, one the the right, for all amino acids: ...
4
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1answer
90 views

What does an output of -I for all amino acids mean in a psi-blast pssm matrix file?

I have run psi-blast using the NR database, remotely, with one iteration, for several sequences to calculate an evolutionary profile (PSSM) for each of those sequences. However, many of the PSSM ...