Questions tagged [qpcr]

The tag has no usage guidance.

Filter by
Sorted by
Tagged with
1
vote
1answer
54 views

How to calculate RNA copies in qPCR using R?

I have these Ct data from qPCR for a series of samples A-H, I want to quantify the RNA copies of my samples using R: ...
1
vote
0answers
152 views

Why is there a minus in the 2ˆ(–delta delta CT) method (qPCR)

Question: Why is there a minus in the 2ˆ(–delta delta CT) formula? My line of thoughs: Consider Ct values of some pPCR ...