Questions tagged [qpcr]
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Feasibility of Enrichment Analysis from RT-qPCR results
Cross-posted on biostars: https://www.biostars.org/p/9581891/
I was wondering if make sense to perform an Enrichment Analysis from results obtained by RT-qPCR. I know you tipically do this with some ...
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Choosing the best number of species for assay
I am doing amplicon sequencing of a virus across many different regions. Lets say I have 20k unique variants of the same virus that I put into my pcr assay and after sequencing and amplifications I am ...
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What statistics can I use on qPCR data composed of several runs if all normality tests fail on the dataset?
I have >100 qPCR experiments that I'd like to analyze together, each containing the same set of genes (10 genes of interest and 2 reference genes).
I have four different samples (untreated & 3 ...
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How to calculate RNA copies in qPCR using R?
I have these Ct data from qPCR for a series of samples A-H, I want to quantify the RNA copies of my samples using R:
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Why is there a minus in the 2ˆ(–delta delta CT) method (qPCR)
Question: Why is there a minus in the 2ˆ(–delta delta CT) formula?
My line of thoughs:
Consider Ct values of some pPCR ...
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