Questions tagged [sequence]
The sequence tag has no usage guidance.
19
questions
2
votes
1
answer
58
views
Sanger sequencing annotation error
I am a student in a Cancer lab. Working with sanger is new to me. While analyzing a report we found an insertion that has not been reported in any databases so far, we were working on checking if the ...
- 21
3
votes
1
answer
49
views
Downloading genes from NCBI in fasta format
I'm pretty new in bioinformatics.
I need to download FASTA sequences of several genes. The list of genes I have assembled consists of 140 genes, so I'd rather do this through via code than download ...
- 31
1
vote
1
answer
62
views
finding matching motifs in a sequence and their position
I am trying to find some matching motifs on a sequence, as well as the position that the motif is located in and then output that into a fasta file. The code below shows that the motif ...
- 133
2
votes
0
answers
31
views
Is there a way antiSMASH can be used to screen a large number of genomes just for one type of biosynthetic gene cluster?
When we use antismash command with the genome sequence file as follows:
antismash GCA_002095535.1_ASM209553v1_genomic.gbff
it screens the genome for all the ...
- 21
1
vote
3
answers
414
views
How can I remove FASTA sequences with an asterisk inside the sequence?
I have several protein sequences with an asterisk in the middle of the sequence, and I'd like to remove those sequences.
In the example below, I have three sequences:
From this example, I would like ...
- 141
1
vote
1
answer
38
views
Problem with sequence alignment
I'm trying to find if the peptides in column A are present within the longer peptides of column B.
Pairwise alignment tools take the data from both columns as one string (i.e., TIIDYTNNHYTNNHLEII... ...
1
vote
0
answers
36
views
Identification of unique chains in proteins by sequence similarity
I'm trying to find unique chains or proteins within their PDB files. Many proteins have multiple chains, but very often they are identical (say, the PDB file consists of 4 homodimers, for example, so ...
- 21
2
votes
1
answer
41
views
How to get coding sequences (CDS) of UniProt reference proteomes?
I downloaded the following reference proteomes:
https://ftp.ebi.ac.uk/pub/databases/reference_proteomes/QfO/
...
- 151
0
votes
1
answer
43
views
How do I generate sequence logo of ATGC against each position in R?
I am new in R sorry for the naive question. I want to generate the seqlogo for each position using R. Can anyone guide me? Thank you so much in advance.
...
- 11
1
vote
0
answers
10
views
How to graph sequence ranges, for example intron sequences or conserved domains
I am a bioinformatics beginner.
I want to draw line graphs such that for a gene, say gene_A, intronic regions are (20,100), (500,600) and a conserved domain was found in (400-550), then I should get ...
- 21
1
vote
0
answers
19
views
How to interpret complete genome sequence in NCBI
I have a very basic and quick question: does the complete sequence of nucleotides of an organism (for example this one that I am considering in my homework of Pyrococcus Fuoriosus) reported in NCBI ...
2
votes
1
answer
48
views
How to know if ID correspond to nucleotide sequence or protein with a script
I have a text file that contains a list of IDs (314 sequences):
AVP78031.1
AVP78042.1
ATO98108.1
ATO98120.1
ATO98132.1
...
My goal is to make a script (maybe using ...
- 185
1
vote
0
answers
47
views
How to use CD-HIT to filter protein sequences dataset by certain similarity threshold?
I am looking at using CD-HIT to efficiently filter my protein sequences dataset by a similarity threshold of 70% (cut-off). More precisely, what I want to achieve is that for all the remaining ...
- 11
3
votes
0
answers
30
views
Template files for bioattributes and meta-data [closed]
I am in process of submiting my FASTQ files to the SRA database. I am a little confused about how to fill the biosample attributes and SRA metadata.
I would really appreciate if you anyone could share ...
- 185
-2
votes
2
answers
57
views
get sequences begining with TA [closed]
I have a fasta file and the sequences in them are arranged like this:
...
- 133
2
votes
1
answer
589
views
Remove Redundant Sequences from FASTA file in Python
I'm attempting to remove redundant sequences from a fasta file (from NCBI). When I execute this code, it returns the number of spots, not the number of sequences. (Number of spots: 408,293, Number of ...
- 53
5
votes
3
answers
3k
views
How to translate amino acid sequences to Nucleotide sequences
I want to convert a list of fasta ( protein sequences) in a .text file into corresponding nucleotide sequences. A Google search gives me result of DNA to protein conversion but not vice versa. Also, I ...
- 51
2
votes
1
answer
90
views
Infer the new (Ban et. al) ribosomal nomenlature (ex. uL53 ) from the ribosomal protein's sequence
I'm trying to programmatically construct a name for each protein subchain in any ribosome from Uniprot in accordance with Ban et. al's 2014 proposal (excerpt given below) using PDB's and Uniprot's ...
- 322
2
votes
1
answer
213
views
Appropriate tool or algorithm for sloppy alignment of degenerate bases
I have an optimization problem where I have a degenerate nucleotide sequence I want to align against subsets of a reference genome (exons, specifically, to make the problem more tractable).
The ...
- 3,075