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2 votes
3 answers
5k views

Seurat: Error in FetchData.Seurat(object = object, vars = unique(x = expr.char[vars.use]), : None of the requested variables were found:

I was trying to do subsetting via: pbmc = subset(pbmc, subset = nFeature > 1000 & nCount > 4000) R returns: ...
Joy's user avatar
  • 21
0 votes
2 answers
10k views

Subsetting from seurat object based on orig.ident?

I am pretty new to Seurat. I want to subset from my original seurat object (BC3) meta.data based on orig.ident. however, when i use subset(), it returns with Error. ...
Najeha Mohamed's user avatar
3 votes
1 answer
1k views

Subsetting of object existing of two samples

I have a Seurat object existing of an aggregate of two samples namely; RD1 and RD2. I am trying to make a subset of each sample. But it's not really working. I manage to make a subset of the cells but ...
ageans's user avatar
  • 131