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Questions tagged [tdtomato]

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2 answers

Can anyone share insights or provide the complete mRNA sequence for tdtomato-WPRE for scRNA seq analysis?

I'm currently immersed in research involving the Rosa26-tdtomato (Ai9) mouse line. We performed scRNA seq and analysis focusing on tdtomato-expressing cells but faced a challenge with a lower-than-...
Outlander's user avatar
2 votes
5 answers

Low custom tdtomato gene content

I have a set of scRNA-seq samples expressing TdTomato, which has high content in microscope. I followed the 10x cellranger pipelines to finsh the work, my procedures are as follows: added TdTomato on ...
sophia's user avatar
  • 39
5 votes
1 answer

No counts for added gene in cellranger (scRNA-seq)

I have a set of scRNA-seq samples enriched with FACS for cells expressing a specific gene reporter (TdTomato). In particular the gene I want to report has positive counts in the resulting matrix for ...
gc5's user avatar
  • 1,813