Questions tagged [tdtomato]
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2
questions
2
votes
4
answers
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Low custom tdtomato gene content
I have a set of scRNA-seq samples expressing TdTomato, which has high content in microscope. I followed the 10x cellranger pipelines to finsh the work, my procedures are as follows:
added TdTomato on ...
5
votes
1
answer
801
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No counts for added gene in cellranger (scRNA-seq)
I have a set of scRNA-seq samples enriched with FACS for cells expressing a specific gene reporter (TdTomato). In particular the gene I want to report has positive counts in the resulting matrix for ...
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