Questions tagged [tx2gene]

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tximport txidcol and geneidcol selecting wrong column

I'm trying to run a differential expression analysis of 2 samples of the same species, but with different treatments using DESeq2. I'm using Ensembldb to create a tx2gene dataframe for tximport, ...
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tx2gene file for tximport issue

I am trying to use tximport to get raw count matrices of a bulk RNA-seq sequencing experiment. I followed the tutorial:
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