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Nikita Vlasenko
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So, we figured it out. If you delete everything until the [Data] tag in the sample sheet, leaving the [Data] tag, it starts working. Here is the full sample_sheet.csv that is working:

[Data]
Lane,Sample_ID,Sample_Name,index,Sample_Project
1,SI-GA-B4_1,17R,ACTTCATA,Chromium_20180409
1,SI-GA-B4_2,17R,GAGATGAC,Chromium_20180409
1,SI-GA-B4_3,17R,TGCCGTGG,Chromium_20180409
1,SI-GA-B4_4,17R,CTAGACCT,Chromium_20180409
1,SI-GA-B5_1,19RL,AATAATGG,Chromium_20180409
1,SI-GA-B5_2,19RL,CCAGGGCA,Chromium_20180409
1,SI-GA-B5_3,19RL,TGCCTCAT,Chromium_20180409
1,SI-GA-B5_4,19RL,GTGTCATC,Chromium_20180409
2,SI-GA-B4_1,17R,ACTTCATA,Chromium_20180409
2,SI-GA-B4_2,17R,GAGATGAC,Chromium_20180409
2,SI-GA-B4_3,17R,TGCCGTGG,Chromium_20180409
2,SI-GA-B4_4,17R,CTAGACCT,Chromium_20180409
2,SI-GA-B5_1,19RL,AATAATGG,Chromium_20180409
2,SI-GA-B5_2,19RL,CCAGGGCA,Chromium_20180409
2,SI-GA-B5_3,19RL,TGCCTCAT,Chromium_20180409
2,SI-GA-B5_4,19RL,GTGTCATC,Chromium_20180409
3,SI-GA-B4_1,17R,ACTTCATA,Chromium_20180409
3,SI-GA-B4_2,17R,GAGATGAC,Chromium_20180409
3,SI-GA-B4_3,17R,TGCCGTGG,Chromium_20180409
3,SI-GA-B4_4,17R,CTAGACCT,Chromium_20180409
3,SI-GA-B5_1,19RL,AATAATGG,Chromium_20180409
3,SI-GA-B5_2,19RL,CCAGGGCA,Chromium_20180409
3,SI-GA-B5_3,19RL,TGCCTCAT,Chromium_20180409
3,SI-GA-B5_4,19RL,GTGTCATC,Chromium_20180409
4,SI-GA-B4_1,17R,ACTTCATA,Chromium_20180409
4,SI-GA-B4_2,17R,GAGATGAC,Chromium_20180409
4,SI-GA-B4_3,17R,TGCCGTGG,Chromium_20180409
4,SI-GA-B4_4,17R,CTAGACCT,Chromium_20180409
4,SI-GA-B5_1,19RL,AATAATGG,Chromium_20180409
4,SI-GA-B5_2,19RL,CCAGGGCA,Chromium_20180409
4,SI-GA-B5_3,19RL,TGCCTCAT,Chromium_20180409
4,SI-GA-B5_4,19RL,GTGTCATC,Chromium_20180409

It is very unfortunate and disappointing that 10X website is generating wrong sample sheet. Still we got very low mapping rates and trying to figure out what went wrong.

Update

I was supplying --sample-sheet= property, but it should be --samplesheet=. Now it is running fine (except for very low mapping rates) with all the headers on.

Update

It turned out that we were given the wrong indexes, so we made a sample sheet using sample sheet generator again and even with --samplesheet= correct parameter it was not running. Convertion of CLRF with perl or tr did not help. However, concise format of the sample sheet worked out well which looks like the following:

Lane,Sample,Index
1-4,17R,SI-GA-B8
1-4,19RL,SI-GA-B9

It needs to be supplied with --csv= parameter.

Nikita Vlasenko
  • 2.6k
  • 3
  • 27
  • 38