I have `RNA-seq` for two groups of patients: `Responders` to chemotherapy (n=9) versus `non-responders` to chemotherapy (n=24) I have done `DESeq2` and obtain `fold change of responders versus non-responders` in which `positive fold change` means `down-regulation in responders` (if I am not wrong) > head(df) gene FC 1 286499 7.116071 2 260436 6.691189 3 2267 5.751322 4 5968 5.522079 5 4103 5.328008 6 442709 5.036087 > I have done `GSEA` in `pre-ranked` way got this plot for `KRAS pathway` [![enter image description here][1]][1] [1]: https://i.sstatic.net/mwPGW.png I interpret this as; `KRAS pathway` in being `down regulated` in `responders` Am I right? Please correct me