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d_kennetz
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this is the swift standard primer file. You are a bit off in your interpretation of the columns (seems like you missed one as there are 12 columns, not 11 which threw everything off):

  1. Chromosome

  2. Target Start

  3. Target Stop

  4. Target Name (change which affected everything downstream in counting)

  5. 5' Primer Start

  6. 5' Primer Stop

  7. 5' Primer Name

  8. 3' Primer Start

  9. 3' Primer Stop

  10. 3' Primer Name

  11. 5' Primer Sequence

  12. 3' Primer Sequence

To convert to BEDPE you only will need the first 4 columns, and the last letter of the Region name denotes the strand orientation of the region. For example Region 1 has _F denoting forward or (+) strand for BEDPE usage, while region 2 has _R in name denoting reverse or (-) strand for BEDPE.

d_kennetz
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